PMID: 9194750Jun 1, 1997Paper

Acetylated LDL endocytosis by the human monocytic Mono Mac 6sr cells is not mediated by the macrophage type I and II scavenger receptors

Arteriosclerosis, Thrombosis, and Vascular Biology
R ScheitheN Hrboticky

Abstract

We recently reported that the human monocytic Mono Mac 6sr cell line constitutively takes up and degrades acetylated (acLDL) and oxidized LDL through receptor-specific pathways. The present studies were undertaken to further characterize the acLDL binding site on a functional and molecular basis. The degradation of acLDL increased during differentiation of Mono Mac 6sr cells with lipopolysaccharide (10 ng/mL, 72 hours) and low concentrations of phorbol 12-myristate 13-acetate (PMA; 0.1 to 1.0 ng/mL, 72 hours). Higher doses of PMA (5 or 10 ng/mL), however, decreased acLDL degradation. Scatchard plots of acLDL binding in untreated and LPS-differentiated Mono Mac 6sr cells were nonlinear and suggested the presence of more than one binding site. Although the ligand specificity of the acLDL receptor in Mono Mac 6sr cells resembles that of the macrophage type I and type II scavenger receptors, we did not detect mRNA of either receptor type in untreated or differentiated Mono Mac 6sr cells by means of Northern blotting and reverse transcription polymerase chain reaction. Furthermore, ligand blotting with 125I-acLDL failed to detect the 220-kD types I and II scavenger receptor protein. Thus, Mono Mac 6sr cells express an acLDL receptor...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K S MoultonC K Glass
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A MatsumotoH Suzuki
Dec 1, 1989·Analytical Biochemistry·F Rodríguez-VicoH Ramírez
Sep 25, 1989·Biochimica Et Biophysica Acta·D S LeakeM H Nasr-Esfahani
Jan 1, 1983·Methods in Enzymology·J L GoldsteinM S Brown
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G R Sambrano, D Steinberg
Jul 15, 1994·Biochemical and Biophysical Research Communications·R ScheitheP C Weber
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D W DunneK A Joiner
May 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·Y GengG K Hansson
Jan 1, 1994·Journal of Leukocyte Biology·H W Ziegler-HeitbrockJ G Haas
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

❮ Previous
Next ❯

Citations

Apr 24, 2001·Arthritis and Rheumatism·N SetaD T Yu
Jan 7, 2000·Biochemical and Biophysical Research Communications·A K HeidenthalN Hrboticky
Apr 13, 1999·The American Journal of Physiology·G DraudeC Weber
Jul 10, 2003·Biochemical and Biophysical Research Communications·Dorothea Strobach, Reinhard L Lorenz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.