Acetylation reduces SOX9 nuclear entry and ACAN gene transactivation in human chondrocytes

Aging Cell
Michal Bar OzMona Dvir-Ginzberg

Abstract

Changes in the content of aggrecan, an essential proteoglycan of articular cartilage, have been implicated in the pathophysiology of osteoarthritis (OA), a prevalent age-related, degenerative joint disease. Here, we examined the effect of SOX9 acetylation on ACAN transactivation in the context of osteoarthritis. Primary chondrocytes freshly isolated from degenerated OA cartilage displayed lower levels of ACAN mRNA and higher levels of acetylated SOX9 compared with cells from intact regions of OA cartilage. Degenerated OA cartilage presented chondrocyte clusters bearing diffused immunostaining for SOX9 compared with intact cartilage regions. Primary human chondrocytes freshly isolated from OA knee joints were cultured in monolayer or in three-dimensional alginate microbeads (3D). SOX9 was hypo-acetylated in 3D cultures and displayed enhanced binding to a -10 kb ACAN enhancer, a result consistent with higher ACAN mRNA levels than in monolayer cultures. It also co-immunoprecipitated with SIRT1, a major deacetylase responsible for SOX9 deacetylation. Finally, immunofluorescence assays revealed increased nuclear localization of SOX9 in primary chondrocytes treated with the NAD SIRT1 cofactor, than in cells treated with a SIRT1 inhib...Continue Reading

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Citations

Jun 13, 2016·Current Rheumatology Reports·Mona Dvir-GinzbergAshok Kumar
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Methods Mentioned

BETA
immunoprecipitation
acetylation
transfection
FCS
immunoprecipitation assay
electrophoresis
PCRs
ChIP
Ch
Confocal microscopy

Software Mentioned

NIH imagej
ImageJ
Mascot
proteome discoverer
Image J

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