PMID: 7537728Dec 1, 1994Paper

Acetylcholine activates a K+ conductance permeable to Cs+ in guinea pig outer hair cells

Hearing Research
C ErosteguiR P Bobbin

Abstract

Acetylcholine (ACh), the major neurotransmitter released by efferent nerve fibers in the cochlea, has been shown to activate a Ca(2+)-dependent K+ conductance in outer hair cells (OHCs). Previously we reported that this ACh operated conductance is permeable to Cs+. The purpose of the present study was to characterize further this Cs(+)-permeable channel and its dependency on Ca2+ using isolated OHCs and the patch clamp technique in the whole cell configuration. The changes in the ACh response were examined when Cs+, Ba2+, Cd2+, N-methyl-D-glucamine (NMG+) and tetraethylammonium (TEA+) were placed in the external or internal solutions. Cs+ substituted for K+ in carrying the ACh-evoked Ca(2+)-dependent K+ current. When NMG+/TEA+ was substituted for internal K+ ACh-evoked an inward and an outward current, and Cs+ substituted for external K+ blocked the outward but not the inward current evoked by ACh suggesting it was carried by K+. In the NMG+/TEA+ condition, when the cell was held at different Vh values for an extended period of time, the ACh-induced K+ current rectified. In Ba2+ (3 mM) with zero Ca2+ ACh failed to induce any detectable current and the ACh response slowly recovered from the Ba2+ block, suggesting a block at an i...Continue Reading

References

Apr 22, 1992·Proceedings. Biological Sciences·P A Fuchs, B W Murrow
Nov 1, 1992·Progress in Neurobiology·P A Fuchs
May 22, 1991·Proceedings. Biological Sciences·G D Housley, J F Ashmore
Sep 15, 1988·Hearing Research·J Santos-Sacchi, J P Dilger
Apr 1, 1988·Trends in Pharmacological Sciences·I Wessler
Jan 1, 1986·Hearing Research·M C Liberman, M C Brown
Jun 16, 1971·Nature: New Biology·R P Bobbin, T Konishi

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Citations

Mar 3, 1998·Progress in Neurobiology·P S GuthP Valli
Mar 1, 1997·Hearing Research·A P NenovR P Bobbin
Jun 26, 1998·Hearing Research·G D HousleyP R Thorne
Feb 26, 2014·The Journal of Physiology·Paul Albert Fuchs
Dec 19, 2014·Frontiers in Systems Neuroscience·Eleonora Katz, Ana Belén Elgoyhen
Mar 14, 2002·The Journal of Comparative Neurology·Michael Zidanic
Apr 30, 2005·The Journal of Physiology·María Eugenia Gómez-CasatiEleonora Katz
Jun 17, 1998·Audiology : Official Organ of the International Society of Audiology·D Lima da CostaJ M Aran
Nov 24, 1999·The American Journal of Physiology·T van Den AbbeeleP T Huy

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