Acetylcholine agonists stimulate mitogen-activated protein kinase in oligodendrocyte progenitors by muscarinic receptors

Journal of Neuroscience Research
J N Larocca, Guillermina Almazan

Abstract

Oligodendrocytes, the myelin-producing cells of the central nervous system, express muscarinic acetylcholine receptors (mAChR). Activation of this neurotransmitter receptor by the stable acetylcholine analog carbachol (CCh) triggers transducing events, modulating c-fos expression and cellular proliferation. To elucidate the signal transduction pathways involved in the transmission of these cellular events, we examined the ability of CCh to activate mitogen-activated protein kinase (MAPK) in primary cultures of oligodendrocyte progenitors prepared from newborn rat brain. CCh produced a concentration- and time-dependent increase in MAPK activity (predominantly the p42mapk or ERK2) as determined by in-gel MBP kinase assays. Using the non-selective muscarinic antagonist atropine we determined that MAPK-activation by CCH is mediated by muscarinic receptors. In the presence of PD098059, a specific inhibitor of MAPK kinase (MEK), MAPK activity was blocked. Similarly, the presence of extracellular calcium was required for CCh-mediated MAPK activation. To further elucidate the mechanisms involved in MAPK activation by CCh, the role of PKC was studied. In cells in which protein kinase had been downregulated by chronic treatment with 12-O...Continue Reading

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Citations

Jan 11, 2003·British Journal of Pharmacology·Eduardo Molina-HolgadoGuillermina Almazan
Dec 7, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Elfaridah P FrazierMartin C Michel
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Jun 22, 2019·Cellular and Molecular Neurobiology·Ming ZhangXianghui Zhao
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Aug 8, 2021·International Journal of Molecular Sciences·Megan ChesnutDavid Pamies

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