PMID: 701357Sep 1, 1978Paper

Acetylcholine compartments in mouse diaphragm. Comparison of the effects of black widow spider venom, electrical stimulation, and high concentrations of potassium

The Journal of Cell Biology
A GorioB Ceccarelli

Abstract

We have studied the effects of 25 mM potassium, electrical stimulation of the phrenic nerve, and crude black widow spider venom on the ultrastructure, electrophysiology, and acetylcholine (ACh) contents of mouse diaphragms. About 65% of the ACh in diaphragms is contained in a depletable store in the nerve terminals. The rest of the ACh is contained in a nondepletable store that may correspond to the store that remains in denervated muscles and includes, in addition, ACh in the intramuscular branches of the phrenic nerve. About 4% of the ACh released from the depletable store at rest is secreted as quanta and may come from the vesicles, while 96% is secreted in a nonquantized form and comes from an extravesicular pool. The size of the extravesicular pool is uncertain: it could be less than 10%, or as great as 50%, of the depletable store. K causes a highly (but perhaps not perfectly) selective increase in the rate of quantal secretion so that quanta account for about 50% of the total ACh released from K-treated diaphragms. K, or electrical stimulation of the phrenic nerve, depletes both the vesicular and extravesicular pools of ACh when hemicholinium no. 3 (HC-3) is present. However, most of the vesicles are retained under these...Continue Reading

References

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Citations

Mar 1, 1984·Cellular and Molecular Neurobiology·S R Barry, A Gelperin
Sep 11, 1987·European Journal of Pharmacology·F G Carpenter, C R Woodruff
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·M C Tzeng, S S Tian
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Jan 1, 1990·Neuroscience·F ValtortaB Ceccarelli
Jan 1, 1983·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·E Florey, M E Kriebel
Oct 1, 1985·The Journal of Cell Biology·F Torri-TarelliB Ceccarelli
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Feb 1, 1992·Journal of Neurochemistry·V Adam-Vizi
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L C FritzA Gorio
Jan 23, 2016·Neurochemistry International·Bárbara Campos de AragãoCristina Guatimosim
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Nov 28, 2014·Biomolecular Concepts·Diana G Meza-Aguilar, Antony A Boucard

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