Acetylcholine-gated and chloride conductance channel expression in rat muscle membrane.

The Journal of Physiology
R D Heathcote

Abstract

1. During the differentiation of skeletal muscle, there is a synchronized expression of a number of muscle-specific proteins including the acetylcholine-gated ion channel (AChR). Another muscle-specific ion channel, responsible for chloride conductance, was shown to be expressed in an anticoordinate fashion to AChR. An organ culture system for rat lumbrical muscles was developed to manipulate the expression of these two ion channels. 2. Denervation induced a change in expression of both channels that was mimicked in culture and reversed by direct electrical stimulation. 3. The time course of the disappearance of both channels was similar and started immediately after denervation (chloride conductance) or stimulation (AChR). The time course of the appearance of AChR was delayed several days after denervation and culture but chloride conductance increased immediately upon stimulation. 4. The loss of chloride conductance in muscle cultured in cycloheximide exhibited first-order kinetics, providing an estimate of the half-life (2.3 days) for the chloride conductance channel. This resembled the disappearance of chloride conductance in normal medium, suggesting that synthesis of this channel ceases following denervation. The decrease...Continue Reading

Citations

Nov 27, 2002·Muscle & Nerve·David JacobsonJohn P Adelman
Feb 12, 2014·Biomaterials Science·Xiufang GuoJames Hickman
Oct 1, 1991·Journal of Neurobiology·W J MoodyM Villaz
Oct 20, 1998·Journal of Neurobiology·W J Moody

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