Acetylcholine-induced K+ currents in smooth muscle cells of intact rat small arteries

The Journal of Physiology
T WeideltF Markwardt

Abstract

1. The mechanism of the sustained acetylcholine-induced endothelium-dependent hyperpolarization (EDH) in intact rat small mesenteric arteries prestimulated with noradrenaline (10(-6) M) was investigated by means of the single microelectrode voltage-clamp method. 2. The vascular smooth muscle cells (VSMCs) in this preparation are poorly or even not coupled for the reasons that: (1) the mean input resistance Rlnp of the clamped vascular smooth muscle increases from 120 M omega under control conditions to 440 M omega after application of K+ channel blocking drugs, (2) the voltage relaxation after injection of hyperpolarizing currents has a monoexponential time course and is linearly dependent on Rlnp, and (3) voltage steps induced by current-clamp steps are not transferred to locations in the vascular musculature 120 microns apart from the current injecting microelectrode. 3. Sustained (> 5 min) application of ACh (10(-5) M) hyperpolarized the VSMCs by induction of a hyperpolarizing current. This effect was completely blocked by the inhibitor of the nitric oxide (NO) synthase L-NAME (10(-3) M) but not by the inhibitor of the soluble guanylate cyclase (sGCl) Methylene Blue (MB, 10(-4) M). 4. Application of the NO donor sodium nitro...Continue Reading

Citations

Apr 22, 2008·Journal of Theoretical Biology·Adam KapelaNikolaos M Tsoukias
May 4, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Timea Z BeleznaiKim A Dora
Oct 16, 2004·American Journal of Physiology. Cell Physiology·Carla de Arêdes BrumRomulo Leite
Feb 12, 1998·The American Journal of Physiology·H YokoshikiN Sperelakis
Jan 3, 2001·Medicinal Research Reviews·C E HillS L Sandow
Feb 12, 1998·The American Journal of Physiology·D G Welsh, S S Segal

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