PMID: 6397998Dec 1, 1984Paper

Acetylcholine measurement by high-performance liquid chromatography using an enzyme-loaded postcolumn reactor

Analytical Biochemistry
C EvaJ L Meek

Abstract

Choline oxidase and cholinesterase were found to retain their activity for 1-2 weeks at room temperature while adsorbed to a commercially available anion-exchange cartridge. These enzymes convert acetylcholine to H2O2. Acetylcholine can be measured in tissue extracts by separation at pH 7 on a polymeric reverse-phase high-performance liquid chromatography column, conversion of acetylcholine to H2O2 on a postcolumn enzyme-loaded anion-exchange cartridge, and electrochemical detection of the H2O2 formed.

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