PMID: 6403667Apr 1, 1983Paper

Acetylcholine releases ATP from varicosities isolated from guinea pig myenteric plexus

Journal of Neurochemistry
T D White, M Al-Humayyd

Abstract

The effects of cholinergic agonists and antagonists on the release of ATP from isolated myenteric varicosities were studied using a firefly luciferin-luciferase technique. In a previous study, acetylcholine and nicotine released ATP from isolated myenteric varicosities, whereas the muscarinic agonist bethanechol did not. In the present study, release of ATP by acetylcholine was shown to be Ca2+ dependent. d-Tubocurarine competitively antagonized the release of ATP by either acetylcholine or nicotine. Maximal release of ATP by acetylcholine (10(-3) M) was approximately 24% that observed with the depolarizing drug veratridine (5 X 10(-5) M), suggesting either that not all of the varicosities capable of releasing ATP possess nicotinic receptors or that acetylcholine does not depolarize the varicosities to the degree that veratridine does. Tetrodotoxin slightly but significantly reduced ATP release induced by acetylcholine or nicotine, indicating some involvement of Na+ channels in the release process. Finally, 6-hydroxydopamine pretreatment produced a 48% reduction in the acetylcholine-evoked release of ATP, suggesting that much, but possibly not all, of the ATP release occurs from noradrenergic varicosities present in the prepara...Continue Reading

References

Mar 15, 1978·European Journal of Pharmacology·A Rutherford, G Burnstock
Jul 1, 1978·European Journal of Pharmacology·D P WestfallJ N Rowe
Jan 1, 1971·Physiological Reviews·L B Geffen, B G Livett
Apr 1, 1974·The Biochemical Journal·M J DowdallV P Whittaker
Feb 1, 1966·The Journal of Physiology·G BurnstockM J Rand
Jan 1, 1980·Annual Review of Neuroscience·G Jonsson
Apr 23, 1982·European Journal of Pharmacology·T D White
May 1, 1981·Journal of Neurochemistry·N Morel, F M Meunier
May 1, 1964·The Journal of General Physiology·T NARAHASHIW R SCOTT

❮ Previous
Next ❯

Citations

Jan 1, 1988·Pharmacology & Therapeutics·T D White
Sep 1, 1991·Trends in Pharmacological Sciences·I von Kügelgen, K Starke
Jan 1, 1984·Progress in Neuro-psychopharmacology & Biological Psychiatry·T D White
Aug 1, 1991·Naunyn-Schmiedeberg's Archives of Pharmacology·R BültmannK Starke
Mar 3, 2009·Gastroenterology·Brian D Gulbransen, Keith A Sharkey
Jan 1, 1990·Annals of the New York Academy of Sciences·T D White, W F MacDonald

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.