PMID: 8951441Nov 1, 1996Paper

Acetylcholine response in guinea pig outer hair cells. II. Activation of a small conductance Ca(2+)-activated K+ channel

Hearing Research
A P NenovR P Bobbin

Abstract

The type of K+ channel involved in the acetylcholine (ACh) evoked response (Ksub; sub stands for suberyldicholine) in guinea pig outer hair cells (OHCs) is still uncertain. The present study tests the hypotheses that Ksub is one of the following: a big conductance Ca(2+)-dependent K+ channel (BK), a small conductance Ca(2+)-dependent K+ channel (SK), a KA type of K+ channel, or a Kn type of K+ channel. Patch-clamp technique in the whole-cell mode was used to record from single guinea pig OHCs. ACh (100 microM) was applied to voltage-clamped OHCs and the ACh-induced currents (IACh) were measured. Charybdotoxin (100 nM) had no effect on IACh, while apamin (1 microM) blocked more than 90% of IACh. Lowering the external Ca2+ concentration caused a hyperpolarizing shift of the IACh monitored as a function of the prepulse voltage. Increasing internal Mg2+ (Mgi2+) concentration caused a reduction in the outward IACh without affecting the inward IACh. The Ksub channel was found to be permeable to Cs+. In Cs+ solutions, IACh was 45% of the IACh in K+ solutions. The block of IACh by apamin, the dependence on extracellular Ca2+, the incomplete block of IACh by Cs+, and the ACh-induced Cs+ currents favor the hypothesis that Ksub belongs to...Continue Reading

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Citations

May 4, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·T M MatthewsP A Fuchs
Jul 25, 2007·Journal of the Association for Research in Otolaryngology : JARO·Javier Zorrilla de San MartínPaul A Fuchs
Nov 15, 2001·Hearing Research·D Z HeP Dallos
Oct 22, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Isabelle RouxElisabeth Glowatzki
May 14, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Claude ColomerNathalie C Guérineau
Oct 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jimena BallesteroEleonora Katz
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Douglas E VetterJim Boulter
Feb 26, 2014·The Journal of Physiology·Paul Albert Fuchs
Aug 5, 2006·The Journal of Physiology·Gregory I Frolenkov
Dec 19, 2014·Frontiers in Systems Neuroscience·Eleonora Katz, Ana Belén Elgoyhen
Mar 14, 2002·The Journal of Comparative Neurology·Michael Zidanic
Oct 6, 2017·Journal of Neurophysiology·Lauren A PoppiAlan M Brichta
Apr 30, 2005·The Journal of Physiology·María Eugenia Gómez-CasatiEleonora Katz
Dec 1, 2006·The Journal of Biological Chemistry·Sonja J PyottRichard W Aldrich
Dec 5, 2017·Frontiers in Cellular Neuroscience·Xiaorong Xu ParksJoseph C Holt
Oct 17, 2002·Journal of Neurobiology·Dwayne D Simmons
Nov 24, 1999·The American Journal of Physiology·T van Den AbbeeleP T Huy
Mar 23, 1999·Journal of Neurophysiology·D Z He, P Dallos
Jan 12, 2001·Journal of Neurophysiology·N YoshidaW F Sewell
Dec 15, 2020·Scientific Reports·Pankhuri VyasHakim Hiel
Nov 6, 2007·Hearing Research·Greg A O'Beirne, Robert B Patuzzi
Dec 29, 2010·Hearing Research·Eric Wersinger, Paul Albert Fuchs
Jul 19, 2011·Journal of Physiology, Paris·Ana Belén Elgoyhen, Eleonora Katz

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