PMID: 3748455Aug 4, 1986Paper

Acetylcholinesterase is regulated by action potential generation and not by muscle contractile activity per se in mouse muscle in vitro

Neuroscience Letters
J A PowellN Holmes

Abstract

Dysgenic (mdg/mdg) skeletal muscle of the mouse, grown in primary cell culture, fires action potentials in the absence of contractions, thus allowing analysis of the role of electrical activity (without contraction) on the specific activity and molecular forms of acetylcholinesterase (AChE). Specific activity of AChE was assessed by the spectrophometric method of Ellman (Biochem. Pharmacol., 7 (1961) 88-95) and found to increase by 2-5 times in the active myotubes (contraction and action potentials in normal and action potentials, alone in dysgenic muscle) compared to quiescent muscle. Sucrose density sedimentation analysis of muscle homogenates revealed an increase, by 2-3 times, in the proportion of the asymmetric (16S) molecular form of AChE in active muscle of both genotypes. Thus, electrical membrane activity, and not contraction per se, is directly involved in the regulation of levels of specific activity of and accumulation of the asymmetric (16S) form of AChE in muscle cells in culture.

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L L Rubin
Aug 1, 1973·Journal of Cellular Physiology·J A Powell, D M Fambrough
Mar 1, 1972·Developmental Biology·F Bowden-Essien
Dec 1, 1984·Developmental Biology·S De La PorteJ Koenig
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

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Citations

Jan 1, 1989·Molecular Neurobiology·R Laufer, J P Changeux
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette
May 1, 1991·Journal of Neurochemistry·F M Vallette, J Massoulié

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