PMID: 9187246May 23, 1997Paper

Acetylcholinesterases from Elapidae snake venoms: biochemical, immunological and enzymatic characterization

Biochimica Et Biophysica Acta
Y FrobertJ Grassi

Abstract

We analyzed 45 batches of venom from 20 different species belonging to 11 genera from the 3 main families of venomous snakes (Elapidae, Viperidae and Crotalidae). We found high acetylcholinesterase (AChE) activity in all venoms from Elapidae, except in those from the Dendroaspis genus. AChE was particularly abundant in Bungarus venoms which contain up to 8 mg of enzyme per gram of dried venom. We could not detect acetylcholinesterase activity in any batch of venom from Viperidae or Crotalidae. Titration of active sites with an organophosphorous agent (MPT) revealed that the AChE of all venoms have similar turnovers (6000 to 8000 s(-1)) which are clearly higher than those of Torpedo and mammalian enzymes but lower than that of Electrophorus. AChEs from the venom of elapid snakes of the Bungarus, Naja, Ophiophagus and Haemacatus genera were purified by affinity chromatography. SDS-PAGE analysis and sucrose gradient centrifugation demonstrated that AChE is exclusively present as a nonamphiphilic monomer. These enzymes are true AChEs, hydrolyzing acetylthiocholine faster than propionylthiocholine and butyrylthiocholine and exhibiting excess substrate inhibition. Twenty-seven different monoclonal antibodies directed against AChE fro...Continue Reading

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Citations

Mar 21, 2002·Biochimica Et Biophysica Acta·Patrick MassonOksana Lockridge
Mar 20, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·N Jorge da Silva, S D Aird
Mar 30, 2006·Parasitology·M E SelkirkJ B Matthews
Jun 2, 2012·Bulletin of Environmental Contamination and Toxicology·Mushtaq AhmedAkhlaq Ahmad
Jul 16, 2008·Protein Science : a Publication of the Protein Society·Xingding ZhouJ Sivaraman
Apr 8, 2011·The FEBS Journal·Tse Siang KangR Manjunatha Kini
Jan 24, 2006·Chemico-biological Interactions·Ludovic RenaultPascale Marchot
Jul 25, 2006·Chemico-biological Interactions·Mushtaq AhmedMaria R C Schetinger
Apr 22, 2015·Scientific Reports·Zhirui LiuYonghua Ji
Jan 15, 2005·Chembiochem : a European Journal of Chemical Biology·Stéphanie GodoyAgnès P Girard-Egrot
Feb 8, 2018·Toxins·Suchaya SanhajariyaGeoffrey K Isbister
Sep 29, 2019·Toxins·Tarek Mohamed Abd El-AzizJames D Stockand
Oct 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamad RimaZiad Fajloun
Sep 27, 2005·Advances in Colloid and Interface Science·Agnès P Girard-EgrotLoïc J Blum
Mar 17, 2020·Toxicon : Official Journal of the International Society on Toxinology·Syeda U HashmiSyed A Ali
Nov 26, 2021·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Carmen Vidal

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