Acetylglutamate synthase from Neurospora crassa: structure and regulation of expression

Molecular Microbiology
Y G YuR L Weiss

Abstract

A DNA clone which complemented an arg-14 mutation of Neurospora crassa was isolated by sib selection from a cosmid library (pMOcosX). Southern and restriction-fragment-length polymorphism (RFLP) analysis confirmed that the cloned DNA contained the arg-14 gene. The arg-14 gene was identified as the structural gene for acetylglutamate synthase by immunodepletion of enzyme activity with antibodies prepared against an arg-14 fusion protein and by the thermal instability of acetylglutamate synthase in a temperature-sensitive arg-14 mutant. The fungai acetylglutamate synthase has little sequence homology to its bacterial counterpart, unlike other arginine biosynthetic enzymes. Expression of the arg-14 gene is regulated by cross-pathway control similar to many amino acid biosynthetic genes. However, expression of acetylglutamate synthase occurs throughout the developmental growth cycle, unlike other arginine biosynthetic enzymes.

Citations

Mar 31, 2004·Molecular Genetics and Metabolism·Hiroki MorizonoMendel Tuchman
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Ying XuNicolas Glansdorff
Aug 27, 2005·Plant Physiology and Biochemistry : PPB·Robert D Slocum
Jan 1, 2008·The Journal of Biological Chemistry·Marina CaldaraRaymond Cunin
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Yuting ZhangXiaogang Weng

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