Acetylsalicylate-human serum albumin interaction as studied by NMR spectroscopy--antigenicity-producing mechanism of acetylsalicylic acid

Molecular Immunology
K HonmaY Ishikawa


To discover the antigenicity-producing mechanism of acetylsalicylic acid, the interaction of this drug and relevant salicylic acid with human serum albumin (HSA) has been studied by means of nuclear magnetic resonance (NMR) spectroscopy. The determination of spin-lattice relaxation rates (1/T1) of some protons have revealed that one HSA molecule can bind acetylsalicylate and salicylate up to 80 and 290 molecules, respectively. The hydrolysis rates of acetylsalicylate were greatly enhanced in the presence of HSA, especially when the drug/HSA mole ratio was small. Thus, the esterase-like activity of HSA was verified. This activity of HSA was effectively inhibited by salicylate; the effect was ascribed to the stronger binding affinity of salicylate toward HSA as compared with that of acetylsalicylate. Based on these results, the antigenicity-producing mechanism of acetylsalicylate and salicylate has been discussed.


Nov 26, 1973·Annals of the New York Academy of Sciences·B D Sykes, W E Hull
May 17, 1968·Science·D HawkinsR S Farr
Mar 1, 1969·The Journal of Clinical Investigation·D HawkinsR S Farr
Nov 22, 1967·Journal of the American Chemical Society·C C McDonald, W D Phillips
May 1, 1984·Chemical & Pharmaceutical Bulletin·A TakadateK Uekama
Jun 1, 1984·Chemical & Pharmaceutical Bulletin·K MaruyamaM Iwatsuru
Sep 30, 1936·The Journal of Experimental Medicine·K Landsteiner, J Jacobs


Jul 10, 2012·Biochemical and Biophysical Research Communications·Paolo AscenziMauro Fasano
Dec 3, 2014·Journal of Molecular Graphics & Modelling·Prapasiri Pongprayoon, M Paul Gleeson
Jan 19, 2006·Biopolymers·Anna SułkowskaWiesław W Sułkowski
Mar 22, 2019·Current Medicinal Chemistry·Loris LeboffePaolo Ascenzi

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