Achievement amastigotes of Leishmania infantum and investigation of pathological changes in the tissues of infected golden hamsters

Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology
Sajad RashidiGholamreza Hatam

Abstract

Leishmania infantum is an agent of visceral leishmaniasis (VL). Amastigote form is a more appropriate target for investigations on vaccines, treatment, and diagnosis. This study aimed to achieve the amastigotes of L. infantum in the golden hamster and J774 macrophages and report the pathological changes that occur in the liver and spleen of the hamsters with VL. 4 male golden hamsters were infected with L. infantum promastigotes. After 5 months, the hamsters were euthanized and touch and pathology smears were prepared from the livers and spleens. Then, these tissues were homogenized and centrifuged at 100×g. Supernatants were collected and centrifuged at 2000×g and the pellets were collected. In the next part of our study, J774 macrophages were infected with L. infantum promastigotes. Then, the infected macrophages were ruptured. Centrifuge stages were done same the previous part. The amastigotes were observed in touch and pathology smears. A load of amastigotes in the livers was more than the spleens in both types of smears. Although the livers' structure had undergone pathological changes, the spleens were unchanged. Also, the macrophage infectivity ratio was up to 95%. Our results present a simple and accessible way of achie...Continue Reading

References

Mar 1, 1993·The Journal of Eukaryotic Microbiology·A A PanD McMahon-Pratt
Aug 1, 1996·Tropical Medicine & International Health : TM & IH·N GuptaA K Rastogi
Jul 1, 1997·Transactions of the Royal Society of Tropical Medicine and Hygiene·G R HatamS Ardehali
Jun 29, 1999·Clinics in Dermatology·P Desjeux
Nov 13, 2001·Tropical Medicine & International Health : TM & IH·S L Croft
Jun 14, 2002·Acta Tropica·Ashwini SomannaLashitew Gedamu
May 15, 2004·Research in Microbiology·Luciana FumarolaOlga Brandonisio
Apr 25, 2006·Parasite Immunology·J CarriónC Alonso
Dec 6, 2006·Immunology and Cell Biology·Amanda C Stanley, Christian R Engwerda
Dec 12, 2007·Cellular Microbiology·Thomas Naderer, Malcolm J McConville
Apr 28, 2009·Molecular and Biochemical Parasitology·Annie RochetteBarbara Papadopoulou
Dec 23, 2009·The Journal of Antimicrobial Chemotherapy·Pavel GershkovichKishor M Wasan
May 29, 2012·Infectious Disease Clinics of North America·Hiro Goto, José Angelo Lauletta Lindoso
May 9, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Mostafavi MahshidBamorovat Mehdi
Dec 22, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Bassim I MohammadNasser Ghaly Yousif

❮ Previous
Next ❯

Related Concepts