Achieving the balance: Biphasic effects of genistein on PC-3 cells

Journal of Food Biochemistry
Sule Terzioglu-UsakNur Ozten-Kandas

Abstract

This study examined the response of PC-3 cells to physiological (0.5, 2.5, 5, 10 μM) and pharmacological (50 μM) concentrations of genistein which is a main bioactive compound in soy. Following 48 hr genistein treatment, cell-based assays and genome-wide microarray were performed. It was evidenced that maximal physiologically achievable concentrations of genistein (0.5-10 μM) lead to significant increase in cell viability (p < 0.05) and decrease in migration at 0.5 μM (p = 0.000) and 10 μM (p = 0.001). The highest percentage of apoptotic cells was obtained at 50 μM. Microarray analysis gave the most critical pathways such as cell cycle regulation and proliferation, tumorigenesis, DNA damage and repair, stress response, and apoptosis. Physiological concentrations (≤10 μM) induced activation of CDKs, MAPKs, and RPSKs, while high concentrations of genistein (>10 μM) appeared to have a novel mechanism of action, specifically down-regulating TGF-β by decreasing specifically SMAD 2/3,4 which are in the downstream TGF-β signaling cascade. PRACTICAL APPLICATIONS: This study highlights for the first time that maximal physiologically achievable concentrations of genistein (0.5-10 μM) have proliferative effects evidenced by alterations in...Continue Reading

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Citations

Mar 3, 2020·Frontiers in Genetics·Xiaonan YangYang Dong

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