Achilles-Mediated and Sex-Specific Regulation of Circadian mRNA Rhythms in Drosophila

Journal of Biological Rhythms
Jiajia LiMichael E Hughes

Abstract

The circadian clock is an evolutionarily conserved mechanism that generates the rhythmic expression of downstream genes. The core circadian clock drives the expression of clock-controlled genes, which in turn play critical roles in carrying out many rhythmic physiological processes. Nevertheless, the molecular mechanisms by which clock output genes orchestrate rhythmic signals from the brain to peripheral tissues are largely unknown. Here we explored the role of one rhythmic gene, Achilles, in regulating the rhythmic transcriptome in the fly head. Achilles is a clock-controlled gene in Drosophila that encodes a putative RNA-binding protein. Achilles expression is found in neurons throughout the fly brain using fluorescence in situ hybridization (FISH), and legacy data suggest it is not expressed in core clock neurons. Together, these observations argue against a role for Achilles in regulating the core clock. To assess its impact on circadian mRNA rhythms, we performed RNA sequencing (RNAseq) to compare the rhythmic transcriptomes of control flies and those with diminished Achilles expression in all neurons. Consistent with previous studies, we observe dramatic upregulation of immune response genes upon knock-down of Achilles. ...Continue Reading

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Citations

Sep 9, 2019··Hannah De los SantosKristin P. Bennett

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Datasets Mentioned

BETA
GSE36108
GSE24503
GSE77451
GSE120100

Methods Mentioned

BETA
RNAseq
PCR
RIP-seq

Software Mentioned

Limma
DODR
LimoRhyde
RNAseq by Expectation Maximization ( RSEM )
Stellaris RNA FISH Probe Designer
R
JTK
DAVID
Prosap

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