Achilles tendon characterization in GDF-7 deficient mice

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
B MikicD Tsou

Abstract

Growth/differentiation factors (GDFs) play a significant role in numerous skeletal tissues and processes. Previous work using the brachypod mouse has suggested that GDF-5 affects Achilles tendon composition, ultrastructure, and material behavior, as well as tendon repair. The aim of the present study was to examine the role of a related GDF family member, GDF-7 (BMP-12), in intact tendon by studying the Achilles tendon of genetically engineered knockout mice. Achilles tendons from 16-week-old GDF-7 -/- mice contained 14% less GAG/DNA than did wild type littermates (p = 0.0481), although collagen content was comparable to controls. Quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) results show that GDF-5 was upregulated two-threefold in response to the absence of GDF-7 protein. GDF-6 was also upregulated in knockouts, but to a lesser extent (twofold, p = 0.0013). On an ultrastructural level, GDF-7 deficient Achilles tendons exhibited a shift towards smaller diameter fibrils which resulted in a small but significant reduction in mean fibril diameter (-8%, p = 0.05). GDF-7 deficiency did not noticeably affect the expression of fibrillar collagens (I, III, V) or tendon proteoglycans (decorin, fibromodulin, lumi...Continue Reading

References

Mar 8, 1991·Cell·E Ruoslahti, Y Yamaguchi
May 1, 1991·The Journal of Cell Biology·D R KeeneR E Burgeson
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Apr 1, 1997·BioTechniques·L B CreemersV Everts
Feb 13, 1999·The Journal of Biological Chemistry·R V IozzoI Eichstetter
Apr 7, 1999·Acta Orthopaedica Scandinavica·P Aspenberg, C Forslund
Apr 13, 1999·Cell and Tissue Research·P H Francis-WestC W Archer
Dec 28, 1999·Clinical Orthopaedics and Related Research·J LouP R Manske
Apr 15, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B B YoungD E Birk
Nov 21, 2000·Scandinavian Journal of Medicine & Science in Sports·P Aspenberg, C Forslund
Apr 27, 2001·Medicine and Science in Sports and Exercise·C Forslund, P Aspenberg
Jun 12, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B MikicE B Hunziker
Aug 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S H WindahlG Andersson
Jan 10, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J LouP Manske
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 27, 2002·Connective Tissue Research·R T ClarkB Mikic
Jun 29, 2002·The Journal of Biological Chemistry·Karl J JepsenShukti Chakravarti
Nov 19, 2002·Critical Reviews in Eukaryotic Gene Expression·Laurie K McCauleyThomas J Rosol
Jun 12, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Carina ForslundPer Aspenberg
Jul 8, 2003·The Journal of Pathology·Ruth Chiquet-Ehrismann, Matthias Chiquet
Jul 16, 2003·The American Journal of Sports Medicine·Carina Forslund, Per Aspenberg
Aug 16, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A ChhabraB Mikic
Oct 30, 2003·Annals of Biomedical Engineering·Eric M Darling, Kyriacos A Athanasiou
Jul 10, 2004·Experimental Cell Research·Takeharu MinamitaniKen-ichi Matsumoto
Jul 12, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Fredrik SöderstenKjell Hultenby

❮ Previous
Next ❯

Citations

Dec 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christopher L MendiasJohn A Faulkner
Nov 28, 2006·British Journal of Sports Medicine·S J Warden
Jul 6, 2014·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Judith S BrookDavid W Brook
Feb 11, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Alice H HuangRonen Schweitzer
Sep 6, 2008·Birth Defects Research. Part C, Embryo Today : Reviews·Charles C BanosCatherine K Kuo
Feb 28, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Borjana MikicLouann Bierwert
Apr 7, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Scott A JelinskyHoward Seeherman
Feb 27, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sadanand FulzeleMark W Hamrick
Jun 6, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Borjana MikicLouAnn Bierwert
Dec 2, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Borjana MikicLouAnn Bierwert
Mar 4, 2015·Nature Reviews. Rheumatology·Geoffroy NourissatDelphine Duprez
Nov 18, 2014·Genes & Diseases·Richard N WangLewis L Shi
Feb 7, 2015·Current Reviews in Musculoskeletal Medicine·Evan ShapiroMark Drakos
Apr 19, 2008·Clinical Orthopaedics and Related Research·Pernilla EliassonPer Aspenberg
Jun 7, 2008·Clinical Orthopaedics and Related Research·Eugene FarngDavid R McAllister
Feb 2, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Borjana MikicLouann Bierwert
Feb 28, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Borjana MikicErnst B Hunziker
Jul 11, 2009·Growth Factors·Pernilla EliassonPer Aspenberg
Aug 7, 2018·Hand : Official Journal of the American Association for Hand Surgery·Kunihide MuraokaJeffrey Yao
Feb 24, 2011·International Journal of Dermatology·Aida KhaledMohamed Ridha Kamoun
Apr 12, 2017·FEBS Open Bio·Kazutaka UemuraHiroyuki Kato
Jul 17, 2018·Annals of the New York Academy of Sciences·Spencer T BiancoAlice H Huang
Oct 3, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bhavita Walia, Alice H Huang
Jun 18, 2019·Journal of Tissue Engineering·Ibtesam Rajpar, Jennifer G Barrett
Feb 26, 2020·BMC Biomedical Engineering·Sophia K Theodossiou, Nathan R Schiele

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
B MikicLouann Bierwert
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
B MikicLouann Bierwert
The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
B MikicLouann Bierwert
© 2021 Meta ULC. All rights reserved