PMID: 8460672Mar 1, 1993Paper

Acid and base effects on avian osteoclast activity

The American Journal of Physiology
A CaranoH C Blair

Abstract

Osteoclasts generate a massive acid flux to mobilize bone calcium. Local extracellular acidification by polarized vacuolar-type H(+)-ATPase, balanced by contralateral HCO3-(-)Cl- exchange to maintain physiological intracellular pH, is theorized to drive this process. It follows that extracellular pH, PCO2, or HCO3- concentration ([HCO3-]) should impact bone matrix dissolution. However, the effects on bone resorption of the concentrations of these ions or their transmembrane gradients are unknown. Furthermore, because bone management is a vital process, regulatory feedback may minimize such effects. Thus a complex relationship between bone resorption and pH, PCO2, and [HCO3-] is expected but requires experimental determination. We measured bone resorption by isolated avian osteoclasts while varying these parameters across the physiological range. Bone degradation increased 50% from pH 7.3 to 6.7, whether achieved by changing [HCO3-] (2.3-38 mM) at constant HCO3- or PCO2 (15-190 mmHg) at constant [HCO3-]. However, at constant pH, changing PCO2 and [HCO3-] within physiological limits did not affect bone resorption. In contrast, total HCO3- removal at pH 7.4 reduced bone degradation by rat or avian osteoclasts substantially, confir...Continue Reading

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Citations

Jul 27, 1999·Journal of Biomedical Materials Research·T MatsunagaY Uchida
Mar 7, 1997·The Journal of Biological Chemistry·T NordströmS Grinstein
Dec 17, 2004·American Journal of Physiology. Cell Physiology·Mikiko ItoKen-ichi Miyamoto
Jun 24, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Harry C BlairPaul J Orchard
Sep 15, 2006·American Journal of Physiology. Cell Physiology·Mikiko ItoKen-ichi Miyamoto
Sep 6, 2008·Journal of Cellular Biochemistry·Jonathan D Kaunitz, Dean T Yamaguchi
May 28, 2010·Journal of Cellular Biochemistry·Azza GramounMorris F Manolson
Dec 31, 2011·Journal of Biomechanical Engineering·Hamidreza AlidoustiNeil W Bressloff
Jul 3, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Harry C BlairPaul J Orchard
Mar 7, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John P WilliamsJay M McDonald
Jun 27, 1997·Biochemical and Biophysical Research Communications·J P WilliamsR W Hardy
Apr 6, 2002·Bone·A-V Rousselle, D Heymann
Apr 1, 1995·Kidney International·R J Alpern

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