PMID: 6986164Jan 8, 1980Paper

Acid-base equilibria of the oxidized beta-nicotinamide adenine dinucleotide-pyruvate adduct in the ground and electronically excited states. A proton transfer probe for proteins

Biochemistry
A Gafni

Abstract

Proton transfer reactions of the oxidized NAD+-pyruvate adduct in its electronically excited singlet state were studied in aqueous solution and when bound to several proteins. The ionization constant of the adduct in the excited state was found to differ by several orders of magnitude from its value in the ground state. A rapid deprotonation reaction was found to follow electronic excitation if a suitable proton acceptor is present. The deprotonation is accompanied by a marked change in the fluorescence spectrum of the adduct and is therefore easy to detect and follow. The free adduct in aqueous solution was found to be in equilibrium between open and folded conformations of which only the open one is fluorescent. The equilibrium constant between these two forms was evaluated. A complex dependence of the fluorescence on the pH in the range of 1--7 was found, which originates in a shift of the equilibrium constant toward the open conformation and in protonation of acid groups of the adduct. Interaction of the adduct with four proteins was studied. Each had a different effect on the proton transfer reaction, reflecting differences in the microenvironment of the bound adduct. A possible use of the adduct as an excited-state proton...Continue Reading

References

Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B A Gruber, N J Leonard
Mar 1, 1979·Archives of Biochemistry and Biophysics·W R LawsL Brand
Nov 19, 1968·Biochimica Et Biophysica Acta·G Di Sabato
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C Y LeeN O Kaplan
Jul 1, 1973·Archives of Biochemistry and Biophysics·C Y Lee, J Everse
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J R BarrioG Weber
Jun 1, 1974·Analytical Biochemistry·A Grinvald, I Z Steinberg
Jul 1, 1957·Archives of Biochemistry and Biophysics·R M BURTONN O KAPLAN

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Citations

Aug 7, 1983·Journal of Theoretical Biology·D N McKinley

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