Acid-base transport in isolated rabbit duodenal villus and crypt cells

Scandinavian Journal of Gastroenterology
M A AinsworthJ I Isenberg

Abstract

Duodenal mucosal bicarbonate secretion is an important first line of defense against gastric acid. Studies in the ileum indicate that the secretion originates from the crypt cells, whereas villus cells are mainly absorptive. Data on acid/base transporters along the crypt-villus axis in duodenal epithelia are not available. It was our purpose to identify and compare acid/base transporters in isolated mammalian duodenal villus and crypt cells. The proximal duodenum of rabbits was excised, and duodenal epithelial cells were isolated in five fractions by a modified calcium chelation technique. Intracellular pH (pHi) was measured with a pH-sensitive dye and dynamic fluorescence ratio imaging. In both villus and crypt cells incubated in Hepes buffer, removal of Na+ or addition of amiloride decreased basal pHi and pHi recovery after intracellular acidification, indicating an Na+/H+ exchanger in both cell types. In both cell types acid extrusion rates in bicarbonate-buffered Ringer's solution were significantly higher than in Hepes buffer. The bicarbonate-dependent acid extruder was unaffected by removal of Cl- or addition of amiloride but was blocked by removal of Na+, indicating the presence of a NaHCO3 cotransporter in both villus a...Continue Reading

References

Jan 1, 1989·Methods in Cell Biology·R Y Tsien
Dec 1, 1988·The American Journal of Physiology·G BoyarskyW F Boron
Jan 1, 1986·Annual Review of Physiology·W F Boron
Feb 12, 1987·The New England Journal of Medicine·J I IsenbergM A Koss
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Dec 1, 1993·Scandinavian Journal of Gastroenterology·M A AinsworthO B Schaffalitzky de Muckadell
Mar 1, 1996·Scandinavian Journal of Gastroenterology·M AmelsbergJ I Isenberg

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Citations

Jul 6, 2000·The Japanese Journal of Physiology·M FukudaY Saek
Oct 25, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bi-Guang TuoJon I Isenberg
Aug 4, 1998·The American Journal of Physiology·C S ChewG Flemström
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M RepishtiJ I Isenberg
Feb 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J PraetoriusT Johansen
Feb 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·J PraetoriusT Johansen
Jan 14, 1999·The American Journal of Physiology·Y Akiba, J D Kaunitz

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