Acid Degradable Cationic Galactose-Based Hyperbranched Polymers as Nanotherapeutic Vehicles for Epidermal Growth Factor Receptor (EGFR) Knockdown in Cervical Carcinoma

Biomacromolecules
Yi-Yang PengRavin Narain

Abstract

Strong signaling cascades derived from upregulation and overexpression of growth factors such as the EGF-family (epidermal growth factors) have been crucially related to cancer pathogenesis. Gene silencing techniques to modulate the expression of oncogenes and tumor suppresor genes are a strategy that shows great promise for cancer management but still faces some limitations in the design of biocompatible and effective vectors. In this study, we synthesized, by reversible addition-fragmentation chain transfer (RAFT) polymerization, several acid degradable galactose-based hyperbranched cationic polymers with varying molecular weights (10 to 20 kDa) and compositions with 2-lactobioamidoethyl methacrylamide [LAEMA] and 2-aminoethyl methacrylamide hydrochloride [AEMA] at different ratios (2.0, 1.0, and 0.5). These polymers were then evaluated for their ability to enhance Epidermal Growth Factor Receptor (EGFR) knockdown in cervical carcinoma. All the polymer constructs have enhanced capabilities to condensate siRNA (small interfering RNA), showing low toxicity at higher LAEMA:AEMA ratios (1.0 and 2.0). Western blot assays were conducted to quantify the EGFR expression of each treatment group demonstrating superior gene knockdown ef...Continue Reading

References

Feb 6, 1985·Nature·M B Sporn, A B Roberts
Feb 19, 2000·Gene Therapy·S Li, L Huang
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R I NicholsonM E Harper
Nov 13, 2004·Nature·Jürgen SoutschekHans-Peter Vornlocher
Jan 7, 2006·Gene Therapy·D M DykxhoornJ Lieberman
Mar 21, 2006·Human Gene Therapy·Christopher BaumBoris Fehse
Jul 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sun Hwa KimTae Gwan Park
Jun 2, 2007·Nature Reviews. Drug Discovery·Antonin de FougerollesJudy Lieberman
Sep 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Derek W BartlettMark E Davis
Sep 27, 2007·Journal of Cellular Physiology·Antonella De LucaNicola Normanno
Jan 19, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jordy LutenWim E Hennink
Apr 16, 2008·Expert Opinion on Therapeutic Targets·Chuan HuangQizhi Yao
Jan 31, 2009·Nature Reviews. Drug Discovery·Kathryn A WhiteheadDaniel G Anderson
Sep 8, 2009·Journal of the Royal Society, Interface·Christopher L Grigsby, Kam W Leong
Nov 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maartje G NoordhuisAte G J van der Zee
Dec 17, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stephanie DavidCatherine Passirani
Mar 29, 2011·Pharmacology & Therapeutics·Annette K LarsenAmélie Petitprez
Jan 1, 2010·Topics in Current Chemistry·Antons SizovsTheresa M Reineke
Dec 17, 2011·Bioconjugate Chemistry·Neha BhucharRavin Narain
Apr 17, 2012·Bioconjugate Chemistry·Marya AhmedRavin Narain
Aug 14, 2012·Critical Reviews in Oncology/hematology·Ivan Diaz-PadillaAna Oaknin
Sep 15, 2012·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Sarah R MacEwan, Ashutosh Chilkoti
Dec 25, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Stephanie D SteichenNicholas A Peppas
Aug 18, 2016·Advanced Drug Delivery Reviews·Wanyi Tai, Xiaohu Gao
Dec 20, 2017·ACS Applied Materials & Interfaces·Surendar R BathulaDibyendu Banerjee
May 9, 2016·ACS Biomaterials Science & Engineering·Stephen QuanRavin Narain

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Citations

Oct 19, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pengchong WangJianfeng Xing
Feb 5, 2021·Advanced Drug Delivery Reviews·Will Stuart-Walker, Clare S Mahon
Mar 23, 2021·Macromolecular Rapid Communications·Iuliia MyrgorodskaPierangelo Gobbo
Feb 15, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alireza KavandDelphine Chan-Seng
May 4, 2021·Chemical Reviews·Ramya KumarTheresa M Reineke
Jun 23, 2020·Biomacromolecules·Raut BholakantWei Chen

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