PMID: 9249030Jul 1, 1997

Acid-induced disassembly of glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus occurs below pH 2.0

European Journal of Biochemistry
R ChiaraluceV Consalvi

Abstract

The stability of the hexameric glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus at low pH values has been studied by activity assay, spectroscopic methods, size-exclusion chromatography and ultracentrifugation analysis. The enzyme is exceptionally stable and at pH 2.0 its hexameric assembly is preserved despite the changes observed in its tertiary structure. Below pH 1.7 dissociation into monomers starts and is accompanied by a progressive loss of tertiary interactions. Dissociation intermediate(s) were not detectable. At pH 2.0 the addition of NaCl causes the same structural changes observed upon further addition of protons. The monomeric state of the enzyme at pH 1.0 shows a significant content of native secondary structure and can be unfolded by guanidinium chloride. The role of electrostatic interactions in the high stability of the enzyme structure at low pH values is discussed.

References

Dec 18, 1991·European Journal of Biochemistry·R Jaenicke
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y GotoA L Fink
Apr 10, 1990·Biochemistry·Y GotoA L Fink
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Jan 1, 1987·Progress in Biophysics and Molecular Biology·R Jaenicke
Jan 1, 1968·Advances in Protein Chemistry·C Tanford
Jul 1, 1981·Analytical Biochemistry·M R Eftink, C A Ghiron
Mar 1, 1980·Journal of Biochemistry·S KuramitsuK Nakashima
Sep 1, 1995·Trends in Biochemical Sciences·O B Ptitsyn
Jun 18, 1996·Biochemistry·J AntosiewiczM K Gilson

Citations

Sep 6, 2000·European Journal of Biochemistry·R ChiaraluceE Chiancone
Jan 6, 2009·International Journal of Biological Macromolecules·Marjan SabbaghianMohsen Nemat-Gorgani

Related Concepts

Archaea
Circular Dichroism, Vibrational
Glutamate Dehydrogenase
Hydrogen-Ion Concentration
Osmolality
Tertiary Protein Structure
Protein Folding, Globular

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.