PMID: 10306Nov 10, 1976

Acid-induced phosphorylation of adenosine 5'-diphosphate bound to coupling factor 1 in spinach chloroplast thylakoids.

The Journal of Biological Chemistry
R P Magnusson, R E McCarty

Abstract

Adenosine 5'-diphosphate, bound to coupling factor 1 (CF1) in spinach chloroplast thylakoids, is in part converted to adenosine 5'-triphosphate, upon injection of the thylakoids into strong acids in the dark. Bound phosphate serves as the phosphoryl donor for this uncoupler-insensitive conversion. Exposure of the thylakoids to heat or to urea prior to their injection into acid caused dissociation of ADP and prevents the apparent acid-induced synthesis of ATP. Conformational changes in CF1 may be elicited by acid denaturation which resemble those brought about by the proton electrochemical gradient across thylakoid membranes.

Related Concepts

Magnesium ADP
Adenosine Triphosphate, Chromium Ammonium Salt
Etioplasts
Darkness
Hydrogen-Ion Concentration
Light
Magnesium
Photophosphorylation
Plant Proteins
Plants

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