PMID: 9434155Jan 20, 1998Paper

Acid nuclear extracts as mediators of gene transfer and expression

Biochimica Et Biophysica Acta
M BöttgerV I Vorob'ev

Abstract

In an attempt to demonstrate transfection-active DNA packaging proteins in the cell nucleus, we prepared acid nuclear extracts with perchloric acid and subsequent protein fractions by stepwise acetone precipitation. The original extract and these fractions containing different compositions of nuclear proteins were used as DNA packaging agents. After the formation of complexes between these protein fractions and reporter genes, the addition of these complexes to the cells resulted in high transfection rates. Gel electrophoresis shows that the most active fractions contain histone H1 and HMG17. HMG1 exhibits a smaller activity. This result was confirmed by positive transfection experiments with commercial histone H1. Our results show that the transfection activity of acid nuclear protein fractions and histone H1 is dependent on the presence of calcium.

References

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Citations

May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Danuta BalickiErnest Beutler
Mar 22, 2000·Acta histochemica·S ZaitsevM Böttger
Nov 19, 2009·Drug Design, Development and Therapy·Sante Di Gioia, Massimo Conese
Oct 15, 2013·Advances in Colloid and Interface Science·M Carmen MoránBjörn Lindman
Aug 22, 2015·International Journal of Molecular Sciences·Hua LiWenxue Ma
Jun 30, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohammadi KaouassDanuta Balicki
Dec 10, 1999·Experimental Cell Research·D A DeanL C Smith
Nov 15, 2002·Biochimica Et Biophysica Acta·Tetsuya UedaMichiteru Yoshida
Nov 14, 2014·PLoS Neglected Tropical Diseases·Mélissa Erin KhanNoëlle Doyen
Nov 22, 2005·Expert Opinion on Drug Delivery·Rui ZhouDavid A Dean
Mar 2, 2006·Expert Opinion on Drug Delivery·Vijay I KulkarniRayasa R Murthy
May 24, 2005·Gene Therapy·D A DeanW E Zimmer
Aug 8, 2007·The Biochemical Journal·Kylie M Wagstaff, David A Jans
Aug 18, 2012·Journal of Biomaterials Applications·Hamideh ParhizMohammad Ramezani
Jun 21, 2008·Molecular Biology Reports·Annekathrin HaberlandWolfgang Henke
Apr 21, 1999·Biochimica Et Biophysica Acta·A HaberlandM Böttger
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·U LungwitzA Göpferich
Sep 25, 2014·Biomacromolecules·M Carmen MoránM Pilar Vinardell

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