Acid-Sensing Histidine Kinase With a Redox Switch.

Frontiers in Microbiology
Shinya InadaYoko Eguchi

Abstract

The EvgS/EvgA two-component signal transduction system in Escherichia coli is activated under mildly acidic pH conditions. Upon activation, this system induces the expression of a number of genes that confer acid resistance. The EvgS histidine kinase sensor has a large periplasmic domain that is required for perceiving acidic signals. In addition, we have previously proposed that the cytoplasmic linker region of EvgS is also involved in the activation of this sensor. The cytoplasmic linker region resembles a Per-ARNT-Sim (PAS) domain, which is known to act as a molecular sensor that is responsive to chemical and physical stimuli and regulates the activity of diverse effector domains. Our EvgS/EvgA reporter assays revealed that under EvgS-activating mildly acidic pH conditions, EvgS was activated only during aerobic growth conditions, and not during anaerobic growth. Studies using EvgS mutants revealed that C671A and C683A mutations in the cytoplasmic PAS domain activated EvgS even under anaerobic conditions. Furthermore, among the electron carriers of the electron transport chain, ubiquinone was required for EvgS activation. The present study proposes a model of EvgS activation by oxidation and suggests that the cytoplasmic PAS...Continue Reading

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B AricóR Rappuoli
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A Uhl, J F Miller
Sep 5, 1997·Science·F R BlattnerY Shao
Feb 25, 2000·Journal of Molecular Biology·N Fisher, P R Rich
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 3, 2000·Bioscience, Biotechnology, and Biochemistry·A KatoR Utsumi
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Feb 14, 2003·FEMS Microbiology Letters·Yi-Chyi LaiHwei-Ling Peng
Apr 16, 2003·Molecular Microbiology·Nobuhisa Masuda, George M Church
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Roxana MalpicaDimitris Georgellis
Aug 22, 2007·Molecular Biology and Evolution·Peter J A Cock, David E Whitworth
Nov 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yoko EguchiRyutaro Utsumi
Apr 9, 2009·Bioscience, Biotechnology, and Biochemistry·Junji ItouRyutaro Utsumi
Oct 20, 2009·Structure·Andreas MöglichKeith Moffat
Feb 5, 2010·BMC Microbiology·Xiulin HanTao Lu
Jul 16, 2011·Biochimica Et Biophysica Acta·Vitaliy B BorisovMichael I Verkhovsky
Apr 24, 2012·The FEBS Journal·Poonam SharmaMartijn Bekker
Nov 7, 2014·PloS One·Yuki YamanakaKaneyoshi Yamamoto
May 25, 2015·Advances in Applied Microbiology·Daniela De Biase, Peter A Lund
Aug 16, 2016·Journal of Molecular Biology·Christopher P ZschiedrichHendrik Szurmant
Dec 3, 2016·Nucleic Acids Research·Ingrid M KeselerPeter D Karp
Jul 5, 2017·Journal of Bacteriology·Hrishiraj SenPeter A Lund
May 23, 2018·Nucleic Acids Research·Andrew WaterhouseTorsten Schwede

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Citations

Aug 13, 2021·Frontiers in Molecular Biosciences·Martina PasquaBianca Colonna

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Software Mentioned

Prism
SWISS
MODEL
EvgS
GraphPad

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