Acid-sensing ion channel 1a is involved in ischaemia/reperfusion induced kidney injury by increasing renal epithelia cell apoptosis

Journal of Cellular and Molecular Medicine
Nana SongXiaoqiang Ding

Abstract

Acidic microenvironment is commonly observed in ischaemic tissue. In the kidney, extracellular pH dropped from 7.4 to 6.5 within 10 minutes initiation of ischaemia. Acid-sensing ion channels (ASICs) can be activated by pH drops from 7.4 to 7.0 or lower and permeates to Ca2+ entrance. Thus, activation of ASIC1a can mediate the intracellular Ca2+ accumulation and play crucial roles in apoptosis of cells. However, the role of ASICs in renal ischaemic injury is unclear. The aim of the present study was to test the hypothesis that ischaemia increases renal epithelia cell apoptosis through ASIC1a-mediated calcium entry. The results show that ASIC1a distributed in the proximal tubule with higher level in the renal tubule ischaemic injury both in vivo and in vitro. In vivo, Injection of ASIC1a inhibitor PcTx-1 previous to ischaemia/reperfusion (I/R) operation attenuated renal ischaemic injury. In vitro, HK-2 cells were pre-treated with PcTx-1 before hypoxia, the intracellular concentration of Ca2+ , mitochondrial transmembrane potential (∆ψm) and apoptosis was measured. Blocking ASIC1a attenuated I/R induced Ca2+ overflow, loss of ∆ψm and apoptosis in HK-2 cells. The results revealed that ASIC1a localized in the proximal tubular and co...Continue Reading

References

Dec 2, 2005·Journal of the American Society of Nephrology : JASN·Mariko Hara-Chikuma, A S Verkman
Nov 1, 2006·Biochemical Society Transactions·R Simon, Z Xiong
Nov 23, 2006·Brain : a Journal of Neurology·Giuseppe PignataroZhi-Gang Xiong
Sep 22, 2007·Acta Pharmacologica Sinica·Xie-Chuan WengXiao-Yun Ma
May 23, 2008·American Journal of Physiology. Renal Physiology·Tarek M El-AchkarPierre C Dagher
Aug 15, 2008·American Journal of Physiology. Renal Physiology·Gamika A PrathapasingheKarmin O
Aug 30, 2008·The Journal of Pharmacology and Experimental Therapeutics·Yelenis HerreraJavier Cuevas
Jul 31, 2009·American Journal of Physiology. Renal Physiology·Hiroko SonodaMasahiro Ikeda
Feb 20, 2010·Cell Calcium·Kinga Szydlowska, Michael Tymianski
Dec 14, 2011·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Denis A KomarovValery V Khramtsov
Jan 13, 2012·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Wei HuMei-Ying Lin
Jan 27, 2012·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Tarek M El-Achkar, Xue-Ru Wu
Feb 22, 2012·Journal of the American Society of Nephrology : JASN·Savithri BalasubramanianTerry B Strom
Nov 28, 2012·Wiley Interdisciplinary Reviews. Membrane Transport and Signaling·Zhi-Gang Xiong, Tian-Le Xu
Oct 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tetsuro KusabaBenjamin D Humphreys
Jan 7, 2014·Translational Stroke Research·Zaven O'BryantZhi-Gang Xiong
Jan 15, 2015·Neuropharmacology·Stefan Gründer, Michael Pusch
Sep 1, 2015·OncoTargets and Therapy·Yan LiGang Chen
Jul 13, 2016·The Journal of Biological Chemistry·Ming-Hua LiZhi-Gang Xiong
Apr 22, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Li-Ping YuanGui Ming
Sep 16, 2017·Behavioural Brain Research·Kiara T Vann, Zhi-Gang Xiong
Jan 24, 2018·American Journal of Physiology. Renal Physiology·Siree AsvapromtadaMasahiro Ikeda
Feb 21, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Wei CaoXiaoyu Chen

❮ Previous
Next ❯

Citations

Jan 9, 2021·Journal of Neuroscience Research·Rongjiao ShaoDerong Cui
Jun 15, 2020·Biochemical Pharmacology·Volker HerzigGlenn F King
Jun 3, 2021·International Journal of Molecular Sciences·Nina RuanXiang-Ping Chu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
light microscopy
flow cytometry
confocal microscopy

Software Mentioned

Statistical Package for the Social Sciences
FlowJo
Image Measure

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis