Acid stable protease inhibitor in ascites of ovarian carcinoma

Clinica Chimica Acta; International Journal of Clinical Chemistry
K AkazawaH Mihara

Abstract

In patients with ovarian tumors, a novel protease inhibitor which is very stable in acid (AS-PI, acid stable protease inhibitor) was identified in the ascites and tumor fluid as well as in the urine and plasma. The highest AS-PI activity was observed in the tumor fluid of ovarian carcinomas (10.7 +/- 2.3 U/ml), followed by the ascites of ovarian carcinomas (8.2 +/- 4.2 U/ml). There was a significant difference in activity of the tumor fluid and ascites between malignant and benign tumors (p less than 0.005, p less than 0.05, respectively). The same antigenicity of AS-PI fractionated from ascites of ovarian carcinomas to urinary trypsin inhibitor was identified by double immunodiffusion and neutralization techniques. It migrated in the serum albumin fraction on immunoelectrophoresis. By gel filtration, the AS-PI in the ascites of ovarian carcinomas showed a molecular weight of 70000-80000. Two active components with molecular weights of 61300 +/- 2100 and 73300 +/- 2500 were detected by SDS polyacrylamide gel electrophoresis.

References

Feb 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K HochstrasserK Lempart
Sep 29, 1979·Biochimica Et Biophysica Acta·V Barthelemy-ClaveyJ Mizon
Jan 1, 1976·Methods in Enzymology·H SchiesslerH Fritz
Aug 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K HochstrasserE Werle
Mar 1, 1974·Journal of Biochemistry·H Sumi, S Fujii
Jan 1, 1966·Scandinavian Journal of Clinical and Laboratory Investigation·K Egeblad, T Astrup
Oct 15, 1966·Klinische Wochenschrift·N Heimburger, H Haupt
Jan 1, 1982·Acta Haematologica·N Toki, H Sumi
Jul 26, 1982·Biochimica Et Biophysica Acta·Y TanakaK Sasaki
Sep 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·H Sumi, N Toki
May 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·H J FAARVANG
Feb 1, 1959·Acta Endocrinologica·H J FAARVANG
Jan 1, 1965·Scandinavian Journal of Clinical and Laboratory Investigation·J O ALVSAKER
Sep 1, 1940·The Biochemical Journal·R A Kekwick

❮ Previous
Next ❯

Citations

Sep 15, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·K AkazawaH Mihara
Apr 13, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·M MaruyamaH Mihara
Jul 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·T YamamotoH Mihara
Aug 14, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·E YoshidaR Sakai
Sep 30, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·H SumiH Mihara
Jan 1, 1990·The International Journal of Biochemistry·L Odum

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.