PMID: 8605731Mar 1, 1996Paper

Acidic pH enhances the invasive behavior of human melanoma cells

Clinical & Experimental Metastasis
R Martínez-ZaguilánM J Hendrix

Abstract

As a consequence of poor perfusion and elevated acid production, the extracellular pH (pHex) of tumors is generally acidic. Despite this, most in vitro experiments are still performed at the relatively alkaline pHex of 7.4. This is significant, because slight changes in pHex can have profound effects on cell phenotype. In this study we examined the effects of mildly acidic conditions on the in vitro invasive potential of two human melanoma cell lines; the highly invasive C8161, and poorly invasive A375P. We observed that culturing of either cell line at acidic pH (6.8) caused dramatic increases in both migration and invasion, as measured with the Membrane Invasion Culture System (MICS). This was not due to a direct effect of pH on the invasive machinery, since cells cultured at normal pH (7.4) and tested at acidic pH did not exhibit increased invasive potential. Similarly, cells cultured at acidic pH were more aggressive than control cells when tested at the same medium pH. These data indicate that culturing of cells at mildly acidic pH induces them to become more invasive. Since acid pH will affect the intracellular pH (pHin) and intracellular calcium ([Ca2+]in), we examined the effect of these parameters on invasion. While ch...Continue Reading

References

Nov 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·D C ShunguJ D Glickson
Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L M Matrisian
May 1, 1992·Clinical & Experimental Metastasis·M J Duffy
Jan 2, 1992·International Journal of Cancer. Journal International Du Cancer·T T LahV Turk
Oct 1, 1991·The Journal of Clinical Investigation·J D VassalliD Belin
Jan 1, 1991·British Medical Bulletin·I J Fidler
Sep 1, 1991·British Journal of Cancer·J R Griffiths
Dec 1, 1990·Cancer Metastasis Reviews·W G Stetler-Stevenson
Feb 1, 1991·The American Journal of Physiology·R Martínez-ZaguilánR J Gillies
Apr 1, 1990·Trends in Genetics : TIG·L M Matrisian
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J GilliesR Perona
Jul 1, 1990·Cancer Metastasis Reviews·F R Miller, G H Heppner
Jul 1, 1990·Cancer Metastasis Reviews·M M MareelP De Baetselier
Apr 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·E W Khandjian
Nov 1, 1990·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Z M BhujwallaJ R Griffiths
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D D MosserR I Morimoto
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A SchwartzC Lechene
Jan 1, 1986·Annual Review of Physiology·W H Moolenaar
Oct 10, 1986·Science·E Rozengurt
Jan 1, 1987·Cancer Metastasis Reviews·J F WhitfieldP R Walker
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S M FrischZ Werb
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R Gopalakrishna, S H Barsky
Jan 1, 1986·Annals of the New York Academy of Sciences·D Soifer
Jul 16, 1986·Biochemical and Biophysical Research Communications·M MorissetH Rochefort
Nov 15, 1986·Analytical Biochemistry·R J GilliesM Denton
Mar 1, 1985·European Journal of Cancer & Clinical Oncology·M D KramerV Schirrmacher
Dec 1, 1984·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·J L Wike-HooleyH S Reinhold
Jul 7, 1994·Nature·J D Vassalli, M S Pepper
Jul 1, 1994·The American Journal of Physiology·R J GilliesZ Bhujwalla
Oct 1, 1993·The American Journal of Physiology·T B WiegmannS J Morris
Jan 1, 1993·Breast Cancer Research and Treatment·K TryggvasonC Pyke

❮ Previous
Next ❯

Citations

Apr 5, 2000·Pathology Oncology Research : POR·Y KatoJ M Foidart
Apr 7, 2007·Cancer Metastasis Reviews·Pawel SwietachAdrian L Harris
Apr 4, 2007·Cancer Metastasis Reviews·Robert J Gillies, Robert A Gatenby
Feb 28, 2008·Clinical & Experimental Metastasis·Raymond E MoelleringRobert J Gillies
Aug 24, 2011·Clinical & Experimental Metastasis·Arig Ibrahim HashimRobert J Gillies
Apr 2, 2013·Clinical & Experimental Metastasis·Maymona Al-Husari, Steven D Webb
Dec 7, 2007·Journal of Bioenergetics and Biomembranes·Souad R Sennoune, Raul Martinez-Zaguilan
Oct 5, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Juan WangRui Zhou
Sep 11, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Feng YaoHeng Zhao
Feb 14, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yefei RongWenhui Lou
Jun 7, 2005·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Seok Jin KimMichael J Kelley
Sep 25, 2001·International Journal of Radiation Oncology, Biology, Physics·D M BrizelW Mueller-Klieser
May 20, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P M J McSheehyJ R Griffiths
Apr 1, 1997·Critical Reviews in Oncology/hematology·G V Glinsky
Jun 15, 1999·Molecular Medicine Today·M Ohh, W G Kaelin
Apr 14, 2010·Chemical Reviews·Kristine GlundeZaver M Bhujwalla
Jan 11, 2012·Oncogene·L M R FerreiraJ E Dumont
Jul 1, 2009·The Journal of Biological Chemistry·Twan van den BeuckenBradly G Wouters
Nov 26, 2009·The Journal of Biological Chemistry·Ana Paula D Ano BomJerson L Silva
Jan 3, 2013·The Journal of Biological Chemistry·J Allen BaronValeria C Culotta
Oct 25, 2006·Molecular and Cellular Biology·Andrew J EvansMichael Ohh
May 7, 2013·Annual Review of Biomedical Engineering·David W InfangerClaudia Fischbach
Mar 12, 2009·Cancer Research·Ian F RobeyRobert J Gillies
Jan 5, 2013·Cancer Research·Veronica EstrellaRobert J Gillies
Sep 6, 2013·Cancer Cell International·Yasumasa KatoYuh Baba
Oct 22, 2013·Molecular Cancer·Huijuan WangYing-Lan Zhao
Jun 17, 2010·Journal of Translational Medicine·Veronica HuberStefano Fais
Aug 3, 2012·Nutrition & Metabolism·Ian Forrest Robey
Oct 11, 2005·Neuro-oncology·Martin A ProescholdtMarsha J Merrill
Aug 31, 2013·PloS One·Maymona Al-Husari, Steven D Webb
Nov 3, 2007·Biological Chemistry·Bernadette C Victor, Bonnie F Sloane
Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stephan Joel ReshkinRosa Angela Cardone
Mar 25, 2006·Future Oncology·Angelo De Milito, Stefano Fais
Feb 26, 2010·Journal of Oncology·L D KellenbergerJ Petrik
Aug 13, 2013·BioMed Research International·Ian F Robey, Lance A Nesbit
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ParkkilaW S Sly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.