PMID: 11926991Apr 3, 2002Paper

Acidic phospholipids with unsaturated fatty acids inhibit the binding of origin recognition complex to origin DNA

Journal of Biochemistry
Jong-Ryul LeeT Mizushima

Abstract

Origin Recognition Complex (ORC) is a candidate initiator of chromosomal DNA replication in eukaryotes. We recently reported that cardiolipin inhibits the interaction of Origin Recognition Complex ORC with origin DNA, as is the case of DnaA, the initiator of chromosomal DNA replication in prokaryotes. We report here that another acidic phospholipid, phosphatidylglycerol (PG), also inhibits the interaction. Synthetic PG with only unsaturated fatty acids inhibits ORC-binding to origin DNA more strongly than PG with only saturated fatty acids. On the other hand, phosphatidylcholine (neutral phospholipid) does not affect the ORC-origin interaction, regardless of the presence of saturated or unsaturated fatty acids. These results suggest that an acidic moiety and unsaturated fatty acids are important factors for the inhibitory effect of phospholipids on ORC binding to origin DNA, as is the case for DnaA. The inhibitory effect of cardiolipin on ORC binding to origin DNA was more apparent at 30 degrees C than at 4 degrees C. Furthermore, chlorpromazine restored the ORC-origin interaction in the presence of cardiolipin. Since the presence of unsaturated fatty acids, low incubation temperatures, and the addition of chlorpromazine all de...Continue Reading

Citations

May 30, 2002·Biological & Pharmaceutical Bulletin·Hitomi TakenakaTohru Mizushima
Jun 9, 2004·Journal of Molecular Biology·Hitomi TakenakaTohru Mizushima
Aug 26, 2006·Bulletin of Experimental Biology and Medicine·V A Trofimov, A A Dudko
Apr 9, 2008·The Biochemical Journal·Masaya TakeharaTohru Mizushima
Feb 9, 2005·The Journal of Biological Chemistry·Concetta C DiRussoPaul N Black

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