Acidity-responsive gene delivery for "superfast" nuclear translocation and transfection with high efficiency

Biomaterials
Jing-Yi ZhuXian-Zheng Zhang

Abstract

In principle, not only efficient but rapid transfection is required since it can maximize the bioavailability of vector-carried gene prior to the cellular excretion. However, the "rapid" goal has been paid few attentions so far in the research field of vector-aided transfection. As a pioneering attempt, the present study designed a lysosome-targeting acidity-responsive nanoassembly as gene vectors, which proved the amazing potency to mediate the "Superfast" transnuclear gene transport and gene transfection with high efficiency in vitro and in vivo. The nanoassembly was constructed on the pH-reversible covalent boronic acid-diol coupling between 1,3-diol-rich oligoethylenimine (OEI-EHDO) and phenylboronic acid modified cholesterol (Chol-PBA). The rapid and efficient nuclei-tropic delivery and transfection was demonstrated to highly rely on the lysosome-acidity induced assembly destruction followed by the easy liberation of gene payloads inside cells. The nanoassembly-mediated transfection at 8 h can afford the outcome even comparable to that achieved at 48 h by the golden standard of PEI25k, and the transfection efficiency can still remain at a high level during 48 h. In contrast, time-dependent efficiency enhancement was identi...Continue Reading

References

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Citations

Jan 21, 2017·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Azadeh Hashem NiaMohammad Ramezani
May 3, 2019·Scientific Reports·Hongyi XieHuaming Yang
Aug 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jie ZhaoChao Lin
Dec 29, 2020·Chemical Reviews·Stephanie FuchsMinglin Ma
Jul 27, 2021·Advanced Science·Cheng YuJingzhi Ma
Jul 7, 2021·Nanoscale·Xiaoqian XinXiaowen Liu
Nov 18, 2017·ACS Applied Materials & Interfaces·Yun-Xia SunXian-Zheng Zhang
May 24, 2019·ACS Applied Materials & Interfaces·Qingyan ZhangMinjie Sun
Sep 5, 2018·ACS Applied Materials & Interfaces·Zhanwei ZhouMinjie Sun

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