Acidosis attenuates P2X purinergic vasoconstriction in skeletal muscle arteries

American Journal of Physiology. Heart and Circulatory Physiology
Heidi A KluessPhilip S Clifford

Abstract

Vasoconstriction via alpha(2)-receptors is known to be sensitive to acidic pH, but little is known about the pH sensitivity of P2X receptors. ATP is a cotransmitter released with norepinephrine from the sympathetic nerves and causes vasoconstriction via P2X purinergic receptors on vascular smooth muscle. We hypothesized that reductions in pH would attenuate P2X-mediated vasoconstriction in iliofemoral artery rings. Twenty-five rats were killed, and the iliac and femoral arteries were dissected out and placed in modified Krebs-Henseleit buffer. The arteries were cut into 2-mm sections and mounted in an organ tissue bath. Tension (g) was measured during a potassium chloride and norepinephrine challenge (maximal tension). The arteries were then exposed to alpha,beta-methylene ATP (10(-7)-10(-3) M; n = 13) or phenylephrine (10(-7)-10(-4) M; n = 6) with a tissue bath pH of 7.8, 7.4, and 7.0. Dose-response curves were fit with nonlinear regression analysis to calculate the EC(50) and slope. The peak tension with alpha,beta-methylene ATP was lower during pH 7.0 (1.37 +/- 0.09 g) compared with pH 7.8 (1.90 +/- 0.12 g). EC(50) was highest with pH 7.4 (-5.38 +/- 0.18 log M alpha,beta-methylene ATP) and lowest with pH 7.0 (-4.9 +/- 0.10 l...Continue Reading

References

Apr 1, 1990·Acta Physiologica Scandinavica·N Sjöblom-Widfeldt, H Nilsson
Apr 1, 1996·British Journal of Pharmacology·B F KingG Burnstock
Feb 1, 1997·Pflügers Archiv : European journal of physiology·C LiF F Weight
Aug 1, 1997·British Journal of Pharmacology·B F KingG Burnstock
Oct 27, 1997·Journal of Neurophysiology·R StoopR A North
Aug 26, 1998·Cardiovascular Research·G L SmithS Wray
Feb 23, 1999·Clinical and Experimental Pharmacology & Physiology·H NakanishiJ Kimura
Mar 30, 2001·The Journal of Biological Chemistry·H XuC Jiang
Nov 27, 2002·European Journal of Pharmacology·Airat U ZiganshinGeoffrey Burnstock
Apr 5, 2003·Stroke; a Journal of Cerebral Circulation·Naohiko SantaMitsuo Iida
May 10, 2003·Circulation Research·Xueren WangChun Jiang
May 27, 2003·Journal of Applied Physiology·John B BuckwalterPhilip S Clifford
Sep 2, 2003·Biochimica Et Biophysica Acta·Erik M Schwiebert, Akos Zsembery
Sep 13, 2003·European Journal of Pharmacology·Dileep Kumar RohraYasushi Ohizumi
Oct 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·John B BuckwalterPhilip S Clifford

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Citations

Oct 9, 2012·Journal of Applied Physiology·Stephen J IvesRussell S Richardson
Feb 2, 2010·Journal of Neurosurgical Anesthesiology·Arthur E Schwartz, David C Adams
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
May 14, 2005·Journal of Applied Physiology·Heidi A KluessPhilip S Clifford
Jul 7, 2017·Experimental Physiology·Stephen J IvesRussell S Richardson
Mar 24, 2007·Journal of Applied Physiology·Zhaohui GaoJianhua Li
Jul 17, 2012·Experimental Physiology·Stephen J IvesRussell S Richardson
Apr 18, 2018·Frontiers in Pediatrics·Alexandra Francoise Puchwein-SchwepckeUlrich H Thome

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