Acidosis induces hyperpermeability in Caco-2BBe cultured intestinal epithelial monolayers

The American Journal of Physiology
M J MenconiM P Fink

Abstract

We previously demonstrated that ileal mucosal acidosis in pigs reversibly increases intestinal permeability to hydrophilic macromolecules, even in the absence of tissue hypoxia [A.L. Salzman, H. Wang, P.S. Wollert, T.J. Vandermeer, C.C. Compton, A.G. Denenberg, and M.P. Fink. Am. J. Physiol. 266 (Gastrointest, Liver Physiol, 29): G633-G646, 1994]. In an effort to further explore the mechanism(s) underlying this phenomenon, we examined the effect of acidic pH on the permeability characteristics of cultured Caco-2BBe (human intestinal epithelial) cells grown as monolayers on permeable supports. Permeability was determined by measuring the mucosal-to-basolateral flux of fluorescein disulfonic acid (FS; mol wt 478 Dal, fluorescein isothiocyanate-labeled dextran (FD4; average mol wt 4 kDa), or [3H]mannitol. Incubation of monolayers under hypercarbic conditions or with acidific bicarbonate-free medium significantly increased permeability to FS, FD4, and mannitol in a manner dependent on both time and pH. Incubation in medium at pH 5.43 for 24 h increased the release of lactate dehydrogenase and decreased the intensity of staining with calcein-acetoxymethyl ester, findings that are indicative of plasma membrane injury; nevertheless, t...Continue Reading

Citations

Jun 1, 2018·Journal of Sports Medicine·Lawrence E ArmstrongElizabeth M Armstrong
Sep 27, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·K G DickmanR D Shaw
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Abrar U KhanMitchell P Fink
Nov 12, 2005·American Journal of Physiology. Cell Physiology·Shiguang LiuMitchell P Fink
Jun 6, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kathleen G RamanMitchell P Fink
Aug 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·H B JijonK L Madsen
Nov 16, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chong LiJing-Yan Han
Aug 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A BananA Keshavarzian
Oct 22, 2008·Infection and Immunity·Amber M JohnsonPhilip R Hardwidge

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