Acinar primary cell culture from the digestive gland of Pecten maximus (L.): an original model for ecotoxicological purposes

Journal of Experimental Marine Biology and Ecology
G Le Pennec, M Le Pennec

Abstract

The purpose of this study was to establish an original primary acinar cell culture model for the mollusc bivalve Pecten maximus (L.), and to define its values and limits for subsequent ecotoxicological applications. To prevent microbial contaminations occurring frequently in invertebrate cell cultures, a perfusion of the stomach-digestive gland complex was performed in situ using a sterile salt solution containing broad-range antibiotics. Digestive acini were isolated using a pronase enzyme that was removed by several washings of the acinar suspension, after which their viability and functionality were determined by three different assays: fluorescein diacetate (FDA) de-esterification, 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium (MTT) reduction and neutral red (NR) incorporation describing de-esterification, mitochondrial dehydrogenase and lysosome activity, respectively. The kinetic conditions for these assays were defined beforehand. The results showed that digestive acini could be maintained in vitro both cytologically and functionally for at least 96 h, which is sufficient for many ecotoxicological applications. Preliminary contamination assays, according to the function studied (cell esterases, mitochondrial respir...Continue Reading

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Citations

May 20, 2005·Cell and Tissue Research·Amagoia Gómez-MendikuteMiren P Cajaraville
Sep 1, 2005·Marine Biotechnology·Baruch Rinkevich
Aug 4, 2010·Cytotechnology·Mathilde van der MerweRouvay Roodt-Wilding
Jul 3, 2008·Environmental Monitoring and Assessment·Mohamed BanniHamadi Boussetta
Apr 2, 2005·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Jérôme FaucetThierry Burgeot
May 29, 2013·Ecotoxicology and Environmental Safety·Sudipta ChakrabortySajal Ray
Nov 15, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gaël Le Pennec, Marcel Le Pennec
Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Mar 8, 2018·Cytotechnology·Andrew BarrickAmélie Châtel
May 1, 2012·Molecular Biology Reports·Yanan YouBaozhong Liu

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