Acinetobacter baumannii transfers the blaNDM-1 gene via outer membrane vesicles

The Journal of Antimicrobial Chemotherapy
Somdatta ChatterjeeSulagna Basu

Abstract

To investigate the transmission of the gene encoding New Delhi metallo-β-lactamase-1 ( bla NDM-1 ) through outer membrane vesicles (OMVs) released from an Acinetobacter baumannii strain (A_115). Isolation and purification of OMVs by density gradient from a carbapenem-resistant clinical strain of A. baumannii harbouring plasmid-mediated bla NDM-1 and aac(6')-Ib-cr genes was performed. DNA was purified from the OMVs and used for PCR and dot-blot analysis. Vesicles treated with DNase I and proteinase K were used to transform A. baumannii ATCC 19606 and Escherichia coli JM109 strains. MIC values for the transformants were determined, followed by PCR and restriction digestion of plasmids. PFGE was done for A_115 and transformants of ATCC 19606 and JM109. The A. baumannii strain (ST 1462) released vesicles (25-100 nm) during in vitro growth at late log phase. PCR and dot-blot analysis confirmed the presence of bla NDM-1 and aac(6')-Ib-cr genes in intravesicular DNA. bla NDM-1 and aac(6')-Ib-cr genes were transferred to both the A. baumannii ATCC 19606 and E. coli JM109 recipient cells. The transformation frequency of the purified OMVs was in the range of 10 -5 -10 -6 and gradually reduced with storage of OMVs. The sizes of the plasmi...Continue Reading

References

May 1, 1989·Journal of Bacteriology·D W DorwardR C Judd
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Oct 24, 1998·Systematic and Applied Microbiology·L DijkshoornM Vaneechoutte
Jan 11, 2000·The Journal of Antimicrobial Chemotherapy·O CiofuN Høiby
Sep 30, 2000·Applied and Environmental Microbiology·S YaronK R Matthews
Feb 12, 2005·Emerging Infectious Diseases·Aharon AbboYehuda Carmeli
Oct 1, 2010·Journal of Clinical Microbiology·Paul G HigginsHarald Seifert
Aug 10, 2011·Antimicrobial Agents and Chemotherapy·P EspinalJ Vila
Aug 24, 2011·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Nov 15, 2011·Trends in Microbiology·Patrice NordmannDavid M Livermore
Feb 1, 2012·Antimicrobial Agents and Chemotherapy·Anne BoulangerPatrice Nordmann
Jun 29, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R A BonninP Nordmann
Nov 2, 2013·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Gunturu RevathiLi-Yueh Huang
Feb 25, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rayane RafeiMarie Kempf
Mar 25, 2014·Applied and Environmental Microbiology·Shweta FulsundarKaare M Nielsen
May 6, 2014·The Journal of Antimicrobial Chemotherapy·Fernando PasteranAlejandra Corso
Dec 3, 2014·Microbiological Research·Jana Klimentová, Jiří Stulík
May 18, 2016·Nature Chemical Biology·Lisandro J GonzálezAlejandro J Vila

❮ Previous
Next ❯

Citations

Dec 20, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria S Ramirez, Marcelo E Tolmasky
Mar 22, 2018·Emerging Microbes & Infections·Noha M Elhosseiny, Ahmed S Attia
Oct 26, 2018·Proteomics·Sharon Ben-HurNeta Regev-Rudzki
Feb 1, 2019·Clinical Microbiology Reviews·Wenjing WuZhiyong Zong
May 30, 2019·Antimicrobial Agents and Chemotherapy·Jun-Ichi WachinoYoshichika Arakawa
Feb 2, 2018·FEMS Microbiology Letters·Priscila Dauros-SingorenkoSimon Swift
May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Jun 21, 2020·The Journal of Antimicrobial Chemotherapy·Martina BielaszewskaAlexander Mellmann
Aug 11, 2019·Nature Communications·Carolina LópezAlejandro J Vila
Aug 6, 2019·Frontiers in Microbiology·Faye C MorrisAnton Y Peleg
Apr 9, 2020·Frontiers in Microbiology·Julie C Caruana, Scott A Walper
Mar 27, 2020·Frontiers in Cellular and Infection Microbiology·Christian Rueter, Martina Bielaszewska
Jul 14, 2020·World Journal of Microbiology & Biotechnology·Marco A BrovedanGuillermo D Repizo
Jul 7, 2020·Frontiers in Microbiology·Abraham D Salgado-CamargoMiguel A Cevallos
Nov 13, 2020·Infectious Diseases and Therapy·Reham WasfiOla A Abd El-Rahman
Dec 15, 2020·Frontiers in Cellular and Infection Microbiology·Isadora Filipaki Munhoz da RochaLysangela Ronalte Alves
Apr 17, 2021·Biochemical Society Transactions·Joni Renee WhiteSimon Swift
Jul 3, 2021·International Journal of Molecular Sciences·Federica Dell'AnnunziataGianluigi Franci
Jul 12, 2021·The Lancet Infectious Diseases·Maria F MojicaRobert A Bonomo
Aug 28, 2021·International Journal of Molecular Sciences·Federica Dell'AnnunziataGianluigi Franci
Jul 27, 2021·Antimicrobial Agents and Chemotherapy·Carolina LópezAlejandro J Vila
Nov 6, 2021·Journal of Extracellular Vesicles·Natalia Díaz-GarridoLaura Baldomà

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.