Acinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type IV pilin, and the other one dedicated to O-glycosylation of multiple proteins

Molecular Microbiology
Christian M HardingMario F Feldman

Abstract

Multiple species within the Acinetobacter genus are nosocomial opportunistic pathogens of increasing relevance worldwide. Among the virulence factors utilized by these bacteria are the type IV pili and a protein O-glycosylation system. Glycosylation is mediated by O-oligosaccharyltransferases (O-OTases), enzymes that transfer the glycan from a lipid carrier to target proteins. O-oligosaccharyltransferases are difficult to identify due to similarities with the WaaL ligases that catalyze the last step in lipopolysaccharide synthesis. A bioinformatics analysis revealed the presence of two genes encoding putative O-OTases or WaaL ligases in most of the strains within the genus Acinetobacter. Employing A. nosocomialis M2 and A. baylyi ADP1 as model systems, we show that these genes encode two O-OTases, one devoted uniquely to type IV pilin, and the other one responsible for glycosylation of multiple proteins. With the exception of ADP1, the pilin-specific OTases in Acinetobacter resemble the TfpO/PilO O-OTase from Pseudomonas aeruginosa. In ADP1 instead, the two O-OTases are closely related to PglL, the general O-OTase first discovered in Neisseria. However, one of them is exclusively dedicated to the glycosylation of the pilin-like...Continue Reading

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Citations

Nov 5, 2015·Glycobiology·Wael ElhenawyMario F Feldman
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Apr 17, 2019·Glycobiology·Christian M Harding, Mario F Feldman
Dec 19, 2017·Nature Reviews. Microbiology·Christian M HardingMario F Feldman
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Aug 29, 2019·Proceedings of the National Academy of Sciences of the United States of America·Mario F FeldmanChristian M Harding
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Feb 23, 2019·Nature Communications·Christian M HardingMario F Feldman
Nov 14, 2020·ACS Infectious Diseases·Karen D MoultonDanielle H Dube
Jun 3, 2021·International Journal of Molecular Sciences·Anastasiya A KasimovaYuriy A Knirel
Oct 12, 2021·ACS Infectious Diseases·Jeremy A DukeChristian M Harding

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