Aconitine-induced cardiac arrhythmia in human induced pluripotent stem cell-derived cardiomyocytes

Experimental and Therapeutic Medicine
Feng ZhangChengzhi Lu

Abstract

Pre-clinical evaluation of cardiac dysfunction is important for assessing the safety of traditional or novel medicines due to the universality of potential drug-induced heart failure and irreversible arrhythmia. Aconitine (ACO), a traditionally used anti-pyretic, analgesic and anti-rheumatic drug, has been reported to have arrhythmogenic effects. In the present study, the Real-Time Cellular Analysis Cardio system was applied to evaluate the arrhythmogenic effects of ACO in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The results indicated that ACO is capable of increasing the frequency and decreasing the amplitude of hiPSC-CM contraction in a dose- and time-dependent manner. ACO at 0.25 µM increased the beating rate of hiPSC-CMs by 3.7-fold within 30 min, while 3.0 µM of ACO increased the beating rate by 7.3-fold. The present study also evaluated the potential pro-apoptotic effects of ACO by using caspase-3 and caspase-9 kits. To the best of our knowledge, the present study was the first to record the ACO-induced cardiac arrhythmia of hiPSC-CMsin real-time. The results also indicate that ACO-induced cell death is mediated, at least in part, by caspase-dependent apoptotic pathways.

References

Nov 17, 2011·Assay and Drug Development Technologies·Malin K B JonssonBruno Becker
Nov 19, 2011·Journal of Laboratory Automation·Biao XiYama A Abassi
May 12, 2012·Assay and Drug Development Technologies·Matthew F PetersYvonne P Dragan
Feb 13, 2013·International Journal of Cell Biology·Simone Fulda
Aug 13, 2013·International Journal of Medical Sciences·Yu-hong ZhouHong-li Shan
Jul 25, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sandy EldridgeMyrtle Davis
Sep 23, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Clay W ScottMatthew F Peters
Oct 21, 2015·Phytotherapy Research : PTR·Thomas Y K Chan
Oct 19, 2016·Indian Heart Journal·Sandeep TakRajesh Bajari
Mar 18, 2017·Journal of Pharmacological Sciences·Xiaodong LiJianying Shen

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Methods Mentioned

BETA
Assay
biopsy

Software Mentioned

SPSS
RTCA
RTCA Cardio

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