Acoustic-frequency vibratory stimulation regulates the balance between osteogenesis and adipogenesis of human bone marrow-derived mesenchymal stem cells

BioMed Research International
Xi ChenZong-Ping Luo

Abstract

Osteoporosis can be associated with the disordered balance between osteogenesis and adipogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs). Although low-frequency mechanical vibration has been demonstrated to promote osteogenesis, little is known about the influence of acoustic-frequency vibratory stimulation (AFVS). BM-MSCs were subjected to AFVS at frequencies of 0, 30, 400, and 800 Hz and induced toward osteogenic or adipogenic-specific lineage. Extracellular matrix mineralization was determined by Alizarin Red S staining and lipid accumulation was assessed by Oil Red O staining. Transcript levels of osteogenic and adipogenic marker genes were evaluated by real-time reverse transcription-polymerase chain reaction. Cell proliferation of BM-MSCs was promoted following exposure to AFVS at 800 Hz. Vibration at 800 Hz induced the highest level of calcium deposition and significantly increased mRNA expression of COL1A1, ALP, RUNX2, and SPP1. The 800 Hz group downregulated lipid accumulation and levels of adipogenic genes, including FABP4, CEBPA, PPARG, and LEP, while vibration at 30 Hz supported adipogenesis. BM-MSCs showed a frequency-dependent response to acoustic vibration. AFVS at 800 Hz was the most favorable fo...Continue Reading

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Citations

Mar 25, 2017·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Xi ChenFan He
Jan 26, 2018·International Journal of Molecular Sciences·Lifang HuAirong Qian
Aug 23, 2020·International Journal of Molecular Sciences·Kar Wey YongAlistair C Cowie
Mar 17, 2018·International Journal of Biomaterials·Brooke McClarren, Ronke Olabisi
Dec 14, 2019·Current Stem Cell Research & Therapy·Oznur BaskanEngin Ozcivici

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