Acoustic overexposure increases the expression of VGLUT-2 mediated projections from the lateral vestibular nucleus to the dorsal cochlear nucleus.

PloS One
Matthew BarkerMartine Hamann

Abstract

The dorsal cochlear nucleus (DCN) is a first relay of the central auditory system as well as a site for integration of multimodal information. Vesicular glutamate transporters VGLUT-1 and VGLUT-2 selectively package glutamate into synaptic vesicles and are found to have different patterns of organization in the DCN. Whereas auditory nerve fibers predominantly co-label with VGLUT-1, somatosensory inputs predominantly co-label with VGLUT-2. Here, we used retrograde and anterograde transport of fluorescent conjugated dextran amine (DA) to demonstrate that the lateral vestibular nucleus (LVN) exhibits ipsilateral projections to both fusiform and deep layers of the rat DCN. Stimulating the LVN induced glutamatergic synaptic currents in fusiform cells and granule cell interneurones. We combined the dextran amine neuronal tracing method with immunohistochemistry and showed that labeled projections from the LVN are co-labeled with VGLUT-2 by contrast to VGLUT-1. Wistar rats were exposed to a loud single tone (15 kHz, 110 dB SPL) for 6 hours. Five days after acoustic overexposure, the level of expression of VGLUT-1 in the DCN was decreased whereas the level of expression of VGLUT-2 in the DCN was increased including terminals originatin...Continue Reading

References

Oct 1, 1992·Epilepsy Research·M Pierson, S L Liebmann
Jan 1, 1990·Anatomy and Embryology·C M HackneyJ Kolston
Apr 1, 1989·The Journal of Comparative Neurology·R J Weinberg, A Rustioni
Dec 8, 1988·The Journal of Comparative Neurology·R L Snyder, P A Leake
Jan 1, 1987·Neuroscience·R J Weinberg, A Rustioni
Nov 13, 1972·Brain Research·G N Orlovsky
Nov 1, 1984·The Journal of Comparative Neurology·D M FeketeD K Ryugo
Feb 1, 1982·The American Journal of Anatomy·D B Webster, D R Trune
Jan 2, 1995·The Journal of Comparative Neurology·A M ThompsonG C Thompson
Feb 1, 1995·Journal of Neurophysiology·E D YoungR A Conley
Jan 29, 1996·The Journal of Comparative Neurology·D D Wright, D K Ryugo
Jul 1, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·B E LawnerB A Bohne
Oct 24, 1998·Journal of Electromyography and Kinesiology : Official Journal of the International Society of Electrophysiological Kinesiology·M NakamuraY Handa
Dec 29, 1998·Behavioural Brain Research·D P SutherlandK K Glendenning
Oct 26, 1999·Journal of Basic and Clinical Physiology and Pharmacology·S FreemanH Sohmer
Dec 28, 1999·American Journal of Otolaryngology·R A Levine
Mar 21, 2000·The Journal of Comparative Neurology·S E ShoreR A Altschuler
Mar 12, 2002·The European Journal of Neuroscience·Ruth IzraeliZvi Wollberg
May 3, 2002·Neuroscience Research·Takeshi Kaneko, Fumino Fujiyama
Nov 14, 2002·Experimental Neurology·S M MulyS J Potashner
Sep 19, 2003·Experimental Brain Research·David K RyugoJohn R Doucet
Jan 20, 2004·Journal of Chemical Neuroanatomy·Martin LévesqueAndré Parent
Apr 23, 2004·Trends in Neurosciences·Robert T FremeauRobert H Edwards
Apr 23, 2004·Trends in Neurosciences·Donata Oertel, Eric D Young

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Citations

Feb 13, 2016·Nature Reviews. Neurology·Susan E ShoreBerthold Langguth
Dec 11, 2013·Hearing Research·Neil P M ToddJames G Colebatch
May 15, 2015·Journal of the Association for Research in Otolaryngology : JARO·Yang LiEric D Young
Dec 21, 2014·Cell and Tissue Research·Calvin WuSusan E Shore
Jul 1, 2016·Proceedings of the National Academy of Sciences of the United States of America·Eyal WigdersonYosef Yarom
Jul 31, 2016·Molecular and Cellular Neurosciences·Senthilvelan ManoharRichard Salvi
Mar 23, 2017·Hearing Research·Margarete Anna UeberfuhrMarkus Drexl
Oct 9, 2019·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Michelle Jee Young SuhSung Huhn Kim
May 14, 2016·Journal of Neurophysiology·Daria GenzelPaul R MacNeilage
Jan 26, 2021·Journal of Vestibular Research : Equilibrium & Orientation·Kristina AntonDietmar Basta
Dec 17, 2020·Frontiers in Neurology·Courtney Elaine StewartFaith W Akin

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Methods Mentioned

BETA
dissection
confocal microscopy

Software Mentioned

ImageJ
CAP
pCLAMP
Powerpoint

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