Acoustically modulated biomechanical stimulation for human cartilage tissue engineering

Lab on a Chip
Umesh S JonnalagaddaRahul S Tare

Abstract

Bioacoustofluidics can be used to trap and levitate cells within a fluid channel, thereby facilitating scaffold-free tissue engineering in a 3D environment. In the present study, we have designed and characterised an acoustofluidic bioreactor platform, which applies acoustic forces to mechanically stimulate aggregates of human articular chondrocytes in long-term levitated culture. By varying the acoustic parameters (amplitude, frequency sweep, and sweep repetition rate), cells were stimulated by oscillatory fluid shear stresses, which were dynamically modulated at different sweep repetition rates (1-50 Hz). Furthermore, in combination with appropriate biochemical cues, the acoustic stimulation was tuned to engineer human cartilage constructs with structural and mechanical properties comparable to those of native human cartilage, as assessed by immunohistology and nano-indentation, respectively. The findings of this study demonstrate the capability of acoustofluidics to provide a tuneable biomechanical force for the culture and development of hyaline-like human cartilage constructs in vitro.

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Citations

Mar 5, 2019·Current Pharmaceutical Design·Lingtian WangWeitao Jia
May 2, 2020·Lab on a Chip·Karl OlofssonMartin Wiklund
Sep 15, 2020·Advanced Biosystems·N CastroS Lanceros-Mendez
Mar 3, 2019·The Journal of the Acoustical Society of America·Zaid ShaglwfPeter Glynne-Jones
Nov 18, 2018·Micromachines·Karl OlofssonMartin Wiklund
Jan 21, 2021·Journal of Functional Morphology and Kinesiology·Graziana MonacoMartin J Stoddart
Mar 27, 2021·The Journal of the Acoustical Society of America·Björn HammarströmMartin Wiklund
May 29, 2020·Trends in Analytical Chemistry : TRAC·Yuliang XieTony Jun Huang
Nov 1, 2021·Electrophoresis·Jakub NovotnyThomas Laurell
Oct 5, 2021·Tissue Engineering. Part B, Reviews·Sarah M Cooper, Roshni S Rainbow

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Methods Mentioned

BETA
total hip arthroplasty

Software Mentioned

MATLAB package MPIV
COMSOL multiphysics
COMSOL
MATLAB
HCImage

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