Acousto-optic laser scanning for multi-site photo-stimulation of single neurons in vitro

Journal of Neural Engineering
Bradley E LosavioPeter Saggau

Abstract

To study the complex synaptic interactions underpinning dendritic information processing in single neurons, experimenters require methods to mimic presynaptic neurotransmitter release at multiple sites with no physiological damage. We show that laser scanning systems built around large-aperture acousto-optic deflectors and high numerical aperture objective lenses provide the sub-millisecond, sub-micron precision necessary to achieve physiological, exogenous synaptic stimulation. Our laser scanning systems can produce the sophisticated spatio-temporal patterns of synaptic input that are necessary to investigate single-neuron dendritic physiology.

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Citations

Feb 1, 2012·Journal of Mathematical Biology·Jay Raol, Steven J Cox
Sep 21, 2010·Nature Methods·Eirini PapagiakoumouValentina Emiliani
Mar 3, 2012·The Review of Scientific Instruments·Yafeng LiuShaoqun Zeng
Aug 27, 2015·Lab on a Chip·B Fan, W Li
Jul 21, 2010·Journal of Neural Engineering·Shy Shoham, Karl Deisseroth
May 31, 2011·Journal of Neural Engineering·Sunggu YangValentina Emiliani

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