Acoustofluidic Fluorescence Activated Cell Sorter

Analytical Chemistry
Ahmad Ahsan NawazTony Jun Huang

Abstract

Selective isolation of cell subpopulations with defined biological characteristics is crucial for many biological studies and clinical applications. In this work, we present the development of an acoustofluidic fluorescence activated cell sorting (FACS) device that simultaneously performs on-demand, high-throughput, high-resolution cell detection and sorting, integrated onto a single chip. Our acoustofluidic FACS device uses the "microfluidic drifting" technique to precisely focus cells/particles three dimensionally and achieves a flow of single-file particles/cells as they pass through a laser interrogation region. We then utilize short bursts (150 μs) of standing surface acoustic waves (SSAW) triggered by an electronic feedback system to sort fluorescently labeled particles/cells with desired biological properties. We have demonstrated continuous isolation of fluorescently labeled HeLa cells from unlabeled cells at a throughput of ∼1200 events/s with a purity reaching 92.3 ± 3.39%. Furthermore, 99.18% postsort cell viability indicates that our acoustofluidic sorting technique maintains a high integrity of cells. Therefore, our integrated acoustofluidic FACS device is demonstrated to achieve two-way cell sorting with high puri...Continue Reading

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Citations

May 18, 2016·Analytical Chemistry·Sixing LiTony Jun Huang
Jul 28, 2016·Lab on a Chip·Armaghan FakhfouriAdrian Neild
Sep 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Petra PaièAndrea Bassi
Sep 23, 2018·Electrophoresis·Yanli GongHai Jiang
Nov 28, 2018·Journal of Micromechanics and Microengineering : Structures, Devices, and Systems·Sinem OrbayTony Jun Huang
Jun 25, 2019·Microsystems & Nanoengineering·Mengxi WuTony Jun Huang
May 27, 2020·Nature Methods·Ahmad Ahsan NawazJochen Guck
Nov 21, 2017·Analytical Methods : Advancing Methods and Applications·David B GoHsueh-Chia Chang
May 21, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tiago Santos-FerreiraMarius Ader
Jun 11, 2016·Cytometry. Part B, Clinical Cytometry·C Wyatt ShieldsGabriel P López
Nov 21, 2017·Biomicrofluidics·Gergely SimonAnne L Bernassau
Oct 3, 2018·Biomicrofluidics·Dong Jun LeeTony Jun Huang
Apr 3, 2019·Ultrasonics·Kentaro TaniDaisuke Koyama
May 7, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ce WangXiaodong Wu
Jun 7, 2018·Analytical Chemistry·Byeongyeon KimSungyoung Choi
Dec 19, 2018·Analytical Chemistry·Peng Li, Tony Jun Huang
Nov 23, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ce WangXiaodong Wu

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