Acquired Enamel Pellicle Engineered Peptides: Effects on Hydroxyapatite Crystal Growth

Scientific Reports
Maria Teresa ValenteWalter L Siqueira

Abstract

The aim of this study was to test the hypothesis that duplication/hybridization of functional domains of naturally occurring pellicle peptides amplified the inhibitory effect of hydroxyapatite crystal growth, which is related to enamel remineralization and dental calculus formation. Histatin 3, statherin, their functional domains (RR14 and DR9), and engineered peptides (DR9-DR9 and DR9-RR14) were tested at seven different concentrations to evaluate the effect on hydroxyapatite crystal growth inhibition. A microplate colorimetric assay was used to quantify hydroxyapatite crystal growth. The half-maximal inhibitory concentration (IC50) was determined for each group. ANOVA and Student-Newman-Keuls pairwise comparisons were used to compare the groups. DR9-DR9 increased the inhibitory effect of hydroxyapatite crystal growth compared to single DR9 (p < 0.05), indicating that functional domain multiplication represented a strong protein evolution pathway. Interestingly, the hybrid peptide DR9-RR14 had an intermediate inhibitory effect compared to DR9 and DR9-DR9. This study used an engineered peptide approach to investigate a potential evolution protein pathway related to duplication/hybridization of acquired enamel pellicle's natural...Continue Reading

References

Mar 1, 1979·Journal of Dental Research·E C Moreno, R T Zahradnik
Apr 25, 1992·British Dental Journal·W M Edgar
Jan 1, 1985·Caries Research·H C Margolis, E C Moreno
Feb 1, 1972·The Journal of Physiology·C Dawes
Aug 1, 1970·Archives of Oral Biology·G H Nancollas, M S Mohan
Oct 13, 1995·The Journal of Biological Chemistry·R M LawnL Patthy
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·L A BobekM J Levine
Apr 5, 2000·The Journal of Biological Chemistry·M GilbertP S Stayton
Nov 10, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Sanjeev KrishnaRichard K Haynes
Feb 17, 2007·Annals of the New York Academy of Sciences·Frank G OppenheimEva J Helmerhorst
Nov 13, 2007·Journal of the American Chemical Society·Bernd GroheGraeme K Hunter
Jun 27, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·James Mallet
Mar 10, 2009·Archives of Oral Biology·Walter L Siqueira, Frank G Oppenheim
Feb 23, 2010·PloS One·Paul V AzzopardiGraeme K Hunter
Sep 20, 2011·Journal of Cell Communication and Signaling·Dusa VukosavljevicWalter L Siqueira
Sep 29, 2012·Journal of Dental Research·W L SiqueiraE E McDonald
Jan 9, 2013·International Journal of Molecular Sciences·Jason N ZimmermanWalter L Siqueira
Jun 28, 2015·Journal of Dental Research·Y XiaoW L Siqueira
Nov 23, 2016·Journal of Dental Research·B Bechinger, S-U Gorr
May 18, 2017·Journal of Dental Research·T BasiriW L Siqueira

❮ Previous
Next ❯

Citations

Aug 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nicholas G FischerConrado Aparicio
Jul 26, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fan ZhangJianshu Li

❮ Previous
Next ❯

Software Mentioned

STATHERIN

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.