Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1.

Malaria Journal
C L MackintoshChristopher I Newbold

Abstract

Antibodies targeting variant antigens expressed on the surface of Plasmodium falciparum infected erythrocytes have been associated with protection from clinical malaria. The precise target for these antibodies is unknown. The best characterized and most likely target is the erythrocyte surface-expressed variant protein family Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). Using recombinant proteins corresponding to five domains of the expressed A4 var gene, A4 PfEMP1, the naturally occurring antibody response was assessed, by ELISA, to each domain in serum samples obtained from individuals resident in two communities of differing malaria transmission intensity on the Kenyan coast. Using flow cytometry, the correlation in individual responses to each domain with responses to intact A4-infected erythrocytes expressing A4 PfEMP1 on their surface as well as responses to two alternative parasite clones and one clinical isolate was assessed. Marked variability in the prevalence of responses between each domain and between each transmission area was observed, as wasa strong correlation between age and reactivity with some but not all domains. Individual responses to each domain varied strikingly, with some individuals ...Continue Reading

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Citations

Jul 8, 2011·Journal of the Royal Society, Interface·Maite SeverinsHans Heesterbeek
Sep 5, 2009·The Journal of Clinical Investigation·Thomas E WellemsRick M Fairhurst
Apr 3, 2014·Cellular and Molecular Life Sciences : CMLS·Jo-Anne ChanJames G Beeson
Oct 1, 2011·Proteomics. Clinical Applications·Obinna N NneduAdam Vigil
Feb 19, 2013·Expert Review of Vaccines·James G BeesonFreya J I Fowkes

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Methods Mentioned

BETA
serum collection
flow cytometry
PCR
electrophoresis
protein assay
ELISA
affinity purification

Software Mentioned

Stata

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