Acquisition of Portal Venous Circulating Tumor Cells From Patients With Pancreaticobiliary Cancers by Endoscopic Ultrasound

Gastroenterology
Daniel V T CatenacciIrving Waxman

Abstract

Tumor cells circulate in low numbers in peripheral blood; their detection is used predominantly in metastatic disease. We evaluated the feasibility and safety of sampling portal venous blood via endoscopic ultrasound (EUS) to count portal venous circulating tumor cells (CTCs), compared with paired peripheral CTCs, in patients with pancreaticobiliary cancers (PBCs). In a single-center cohort study, we evaluated 18 patients with suspected PBCs. Under EUS guidance, a 19-gauge EUS fine needle was advanced transhepatically into the portal vein and as many as four 7.5-mL aliquots of blood were aspirated. Paired peripheral blood samples were obtained. Epithelial-derived CTCs were sorted magnetically based on expression of epithelial cell adhesion molecules; only those with a proper morphology and found to be CD45 negative and positive for cytokeratins 8, 18, and/or 19 and 4',6-diamidino-2-phenylindole were considered to be CTCs. For 5 samples, CTCs also were isolated by flow cytometry and based on CD45 depletion. ImageStream was used to determine the relative protein levels of P16, SMAD4, and P53. DNA was extracted from CTCs for sequencing of select KRAS codons. There were no complications from portal vein blood acquisition. We detect...Continue Reading

References

Mar 19, 2004·The New England Journal of Medicine·John P NeoptolemosUNKNOWN European Study Group for Pancreatic Cancer
Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·W Jeffrey AllardLeon W M M Terstappen
Apr 22, 2006·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Shoichi HishinumaSeiji Igarashi
Apr 9, 2008·Journal of Hepato-biliary-pancreatic Surgery·Toshio KuriharaFuminori Moriyasu
Jul 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Steven J CohenNeal J Meropol
Sep 6, 2008·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·J J KloekT M van Gulik
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Johann S de BonoDerek Raghavan
Jul 9, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amanda BlackfordRalph H Hruban
Nov 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Long R JiaoJustin Stebbing
Jan 24, 2012·Cell·Andrew D RhimBen Z Stanger
Apr 5, 2013·Science Translational Medicine·Emre OzkumurMehmet Toner
Sep 14, 2013·Science·Vicki PlaksZena Werb
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Peng LiTony Jun Huang

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Citations

Feb 3, 2016·Future Oncology·Shuang Qin Zhang, Daniel V T Catenacci
Apr 15, 2016·Expert Review of Gastroenterology & Hepatology·Christopher G Chapman, Irving Waxman
Jun 25, 2016·Future Oncology·Emmanuel Coronel, Irving Waxman
Aug 23, 2016·Gastroenterology Clinics of North America·Bradley W Anderson, David A Ahlquist
Jun 25, 2016·Gastroenterology·Sunitha NagrathDiane M Simeone
Jul 20, 2016·Scientific Reports·Morten LapinOddmund Nordgård
Jun 20, 2019·Molecular Oncology·Etienne BuscailCatherine Alix-Panabières
Jul 26, 2017·Journal of Clinical Gastroenterology·Rajat Garg, Tarun Rustagi
Feb 14, 2020·Cell Cycle·Jae W Lee, Gregory L Beatty
Mar 8, 2020·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·Rintaro Hashimoto, Kenneth J Chang
Jun 22, 2018·Oncotarget·Dipesh Kumar YadavTingbo Liang
Apr 18, 2020·Journal of Clinical Gastroenterology·Carlos Roberto Simons-Linares, Prabhleen Chahal
Jul 20, 2016·World Journal of Gastroenterology : WJG·Taisuke ImamuraEigo Otsuji
Oct 22, 2019·Therapeutic Advances in Gastrointestinal Endoscopy·Roberto Oleas, Carlos Robles-Medranda
Jul 26, 2019·ACG Case Reports Journal·C Roberto Simons-Linares
Feb 9, 2016·World Journal of Gastroenterology : WJG·Stefania De Lisi, Marc Giovannini
Jun 20, 2017·Frontiers in Physiology·Michael PimientaStephen Pandol
Dec 22, 2017·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·C KahlertT Welsch
Jun 27, 2018·Endoscopic Ultrasound·Irina Mihaela CazacuManoop S Bhutani
Aug 18, 2018·Endoscopic Ultrasound·Idan Levy, Kenneth F Binmoeller
May 23, 2018·World Journal of Gastroenterology : WJG·Lulu ZhangAlina Stoita
Aug 18, 2018·Endoscopic Ultrasound·Christopher G Chapman, Irving Waxman
Mar 28, 2020·United European Gastroenterology Journal
Jun 4, 2020·Annals of Gastroenterological Surgery·Minako NagaiKota Nakamura
Dec 22, 2016·Endoscopic Ultrasound·Adrian SaftoiuManoop S Bhutani
Jun 8, 2018·Clinical Endoscopy·Jason B Samarasena, Kenneth J Chang
Dec 10, 2019·Current Gastroenterology Reports·Christopher G Chapman, Irving Waxman
Dec 10, 2019·Clinical Endoscopy·Jae Keun Park, Kwang Hyuck Lee
Apr 16, 2020·International Journal of Molecular Sciences·Samuel AmintasEtienne Buscail
Sep 3, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marvin Ryou, Nicholas Stylopoulos
Dec 15, 2020·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Tony C Y PangMinoti V Apte
Feb 24, 2021·Journal of Vascular and Interventional Radiology : JVIR·Bruno DamascelliTshering Dorji
Jan 23, 2018·Advanced Drug Delivery Reviews·Sabine RiethdorfKlaus Pantel

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Methods Mentioned

BETA
surgical resection
proteomic profiling
flow cytometry
biopsy

Software Mentioned

STATA
IDEAS
CellSearch

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