Acquisition of resistance to butyrate enhances survival after stress and induces malignancy of human colon carcinoma cells

Cancer Research
Isabel López de SilanesM Antonia Lizarbe

Abstract

Acquired resistance to apoptosis by tumor cells remains a major obstacle for cancer treatment, and hence the analysis of resistance to apoptosis constitutes a major goal in the development of antitumoral drugs. We have established a butyrate-resistant human colon adenocarcinoma cell line (BCS-TC2.BR2) from nontumorigenic BCS-TC2 cells to analyze whether the acquisition of such phenotype confers resistance to apoptosis and stress. Although BCS-TC2.BR2 cells exhibited a more differentiated phenotype than the parental BCS-TC2 cells, higher butyrate concentrations remained capable of additionally enhancing their differentiation without inducing apoptosis. Survival rates of BCS-TC2.BR2 cells after glucose deprivation and heat shock were higher than those of parental cells, revealing a stress-resistant phenotype. These findings were accompanied by key differences between parental and butyrate-resistant cells in gene expression profiles and the acquisition of in vivo tumorigenicity. In conclusion, cells gaining resistance to an endogenous physiological modulator of growth, differentiation, and apoptosis concurrently acquired resistance to other agents that influence cell survival.

References

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Citations

Jul 27, 2011·Apoptosis : an International Journal on Programmed Cell Death·Juan Ignacio BarrasaM Antonia Lizarbe
Jun 9, 2012·The British Journal of Nutrition·Kim Y C FungDavid L Topping
Oct 12, 2010·The Journal of Biological Chemistry·Jacinta SerpaSérgio Dias
Sep 13, 2007·Molecular Carcinogenesis·Colleen C SpurlingCharles Giardina
Nov 7, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N OlmoM A Lizarbe
Nov 7, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N OlmoM A Lizarbe
Jan 1, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Juan I BarrasaJavier Turnay
Apr 1, 2006·Nutrition and Cancer·Pablo Pérez-RamosM Antonia Lizarbe
Jun 29, 2013·International Journal of Molecular Sciences·Kim Y C FungDavid L Topping
Jun 10, 2016·Oncology Reports·Hyang Ri KangSo Hee Kim
Jun 3, 2017·BioMed Research International·María Antonia LizarbeJavier Turnay
Jan 7, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Roy HajjarManuela M Santos
Feb 7, 2009·Journal of Proteome Research·Kim Y C FungLeah J Cosgrove

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