Acquisition of tumorigenic potential and therapeutic resistance in CD133+ subpopulation of prostate cancer cells exhibiting stem-cell like characteristics

Cancer Letters
Rajnee KanwalSanjay Gupta

Abstract

The role of CD133 (Prominin-1) as a cancer stem cell marker may be useful for therapeutic approaches and prognostication in prostate cancer patients. We investigated the stem-cell-related function and biological features of a subpopulation of CD133+ cells isolated from established primary human prostate cancer cell lines. The CD133+ cells sorted from human prostate cancer 22Rv1 exhibited high clonogenic and tumorigenic capabilities, sphere forming capacity and serially reinitiated transplantable tumors in NOD-SCID mice. Gene profiling analysis of CD133+ cells showed upregulation of markers of stem cell differentiation (CD44, Oct4, SOX9 and Nanog), epithelial-to-mesenchymal transition (c-myc and BMI1), osteoblastic differentiation (Runx2), and skeletal morphogenesis (BMP2), compared to side population of CD133- cells. These cells are highly malignant and resistant to γ-radiation and chemotherapeutic drug, docetaxel. Importantly, a docetaxel-resistant subclone was more enriched in CD133+ cells with significant increase in Runx2 expression, compared to CD133- cells. Furthermore, knockdown of Runx2 in these cells resulted in differential response to chemotherapy, sensitizing them to increased cell death. These results demonstrate t...Continue Reading

References

Apr 29, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S L ChengR Civitelli
Aug 26, 1999·In Vitro Cellular & Developmental Biology. Animal·R M SramkoskiJ W Jacobberger
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Jul 1, 2004·Journal of Cell Science·Gavin D RichardsonAnne T Collins
Oct 19, 2005·Cancer Research·Dong FangMeenhard Herlyn
Dec 3, 2005·Cancer Research·Anne T CollinsNorman J Maitland
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·M E PrinceL E Ailles
Feb 7, 2007·Cancer Research·Chenwei LiDiane M Simeone
Dec 1, 2007·Cell Death and Differentiation·A EramoR De Maria
Jan 19, 2008·Nature·Tobias SchattonMarkus H Frank
Apr 10, 2008·The Prostate·Christopher J ShepherdDavid L Hudson
Dec 3, 2008·Cancer Research·Donald J Vander GriendJohn T Isaacs
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Giulia BertoliniGabriella Sozzi
Oct 12, 2010·Journal of the National Cancer Institute·Anna Maria CalcagnoSuresh V Ambudkar
Aug 18, 2012·The Journal of Pathology·Philipp Grosse-GehlingLeoni A Kunz-Schughart
Nov 20, 2012·Advances in Experimental Medicine and Biology·Denis CorbeilJózsef Jászai
Aug 24, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Clémentine Le MagnenGiulio C Spagnoli
Jul 24, 2014·International Journal of Cancer. Journal International Du Cancer·Xuhui ZhangJane B Lian
Sep 13, 2014·International Journal of Molecular Medicine·Burak Cem SonerGulperi Oktem
Nov 13, 2014·Methods in Molecular Biology·Eleonora PiscitelliIleana Zucchi
Nov 30, 2016·Oncology Letters·Maria Teresa ValentiLuca Dalle Carbonare
Jan 14, 2017·Pathology Oncology Research : POR·Elham KalantariZahra Madjd
Feb 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eswar ShankarSanjay Gupta
Feb 18, 2017·Molecular Cancer·Pramudita R Prasetyanti, Jan Paul Medema
Mar 7, 2018·Journal of Biomedical Science·Ain Zubaidah Ayob, Thamil Selvee Ramasamy

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Citations

Apr 7, 2020·Current Pharmaceutical Design·Qiang LiuZhi-Xiang Yuan
Jan 8, 2019·World Journal of Stem Cells·Mariel E Toledo-GuzmánElizabeth Ortiz-Sánchez
May 31, 2019·Frontiers in Oncology·Gianluca CivenniCarlo V Catapano
May 20, 2020·Frontiers in Molecular Biosciences·Zhengchun WuZhulin Yang
Oct 18, 2019·Journal of Experimental & Clinical Cancer Research : CR·Kotaro SagaYasufumi Kaneda
Oct 14, 2020·Oncogene·Yuanshuo Alice WangNatasha Kyprianou
Dec 9, 2020·International Journal of Molecular Sciences·Jolanta SzenajchLuiza Handschuh
Mar 9, 2021·Signal Transduction and Targeted Therapy·Chaoyue SuLiwu Fu
Mar 25, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Ismail S MohiuddinMin H Kang

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