Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS)

Methods in Molecular Biology
José G VallarinoSonia Osorio

Abstract

Plants synthesize and emit a large range of volatile organic compounds (VOCs) that play important roles in their interactions with the environment, from attracting pollinators and seed dispersers to protectants such as repellants and pathogen inhibitors. As such, the development of techniques for headspace collection of volatiles in combination with gas chromatography-mass spectrometry (GC-MS) has an important impact on our understanding of the biosynthesis of plant VOCs. Furthermore, knowledge of the plant VOCs can be valuable in relation to plant breeding for improving fruit flavor or enhancing resistance to insects or pathogens. This chapter describes a reliable method for extracting volatile compounds by headspace solid-phase microextraction (HS-SPME), and separate and detect them by GC-MS.

Citations

Jul 6, 2019·Scientific Reports·Alexander ErbanJoachim Kopka
Sep 10, 2019·Mass Spectrometry Reviews·Ana Margarida RodriguesCarla António
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shidan WengDongqin Tang
Jun 10, 2021·Journal of Experimental Botany·Diana PimentelAna Margarida Fortes

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