Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods

Acta Veterinaria Hungarica
Jana JankovicováL'ubica Horovská

Abstract

Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed bovine spermatozoa. Double staining and Hoechst/FITC-Pisum sativum agglutinin (FITC-PSA) labelling were tested for evaluating the viability and acrosome reaction induced by calcium ionophore of bull spermatozoa. In our experiments no significant differences were detected in the frequency of acrosome-reacted sperm either by double staining (37.98%) or by FITC-PSA labelling (39.33%). The viability of sperm stained by the double staining method was 67.17%, and a higher portion of viable sperm (82.67%) was observed by staining with the Hoechst procedure (P < 0.01). On the basis of the results obtained it is concluded that both methods can be used for detecting the acrosome reaction of frozen-thawed bovine spermatozoa.

References

May 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A Kovács, R H Foote
Oct 1, 1989·Biology of Reproduction·N L Cross, S Meizel
Dec 1, 1988·The Journal of Experimental Zoology·R Yanagimachi, A Bhattacharyya
Mar 29, 2000·Theriogenology·S NagyR H Foote
Oct 2, 2002·Reproduction in Domestic Animals = Zuchthygiene·T SomfaiA Kovács
Apr 1, 1986·Theriogenology·J J ParrishN L First

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Citations

Mar 13, 2016·Journal of Cellular Biochemistry·Andrea C AguileraMiguel A Sosa
Nov 12, 2016·Reproductive Toxicology·Alisa Komsky-Elbaz, Zvi Roth
Jul 9, 2020·Veterinary World·Zulfi Nur Amrina RosyadaBambang Purwantara
Apr 26, 2021·International Journal of Biological Macromolecules·Benildo Sousa CavadaKyria Santiago Nascimento

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