PMID: 9540823Apr 16, 1998Paper

Acrosome reaction inactivation in sea urchin sperm

Biochimica Et Biophysica Acta
A GuerreroA Darszon

Abstract

Acrosome reaction inactivation (ARI) is a process that renders sperm irreversibly refractory to the egg jelly (the natural inducer of the acrosome reaction, AR). This process triggered by the egg jelly, is associated with an increase in [Ca2+]i. However, we show here that a rise in [Ca2+]i alone is not sufficient to induce ARI, since artificially increasing [Ca2+]i with either an ionophore or rising external pH, does not trigger ARI. Contrary to the AR which strictly requires Ca2+, ARI can be triggered almost equally well by Sr2+. On the other hand, Mn2+ inhibits ARI and, as we showed earlier, does not affect AR. These observations indicate that the mechanisms involved in ARI differ from those leading to AR. In addition, we report here that high external pH (a non-physiological inducer of AR) triggers the AR in previously inactivated sperm by opening the same Ca2+ channels activated by the egg jelly. Considering that the opening of Ca2+ channels is one of the earliest responses triggered by the egg jelly and that ARI requires the egg jelly receptor to be activated, we have concluded that ARI involves the uncoupling between the egg jelly receptor and Ca2+ channels. Furthermore, intracellular pH (pHi) determinations, in the prese...Continue Reading

References

Feb 1, 1990·The Journal of General Physiology·A LiévanoA Darszon
Jan 1, 1987·Annual Review of Biophysics and Biophysical Chemistry·R W TsienR L Rosenberg
Jan 1, 1989·Annals of the New York Academy of Sciences·M FillR Coronado
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J S TrimmerV D Vacquier
Mar 1, 1987·Developmental Biology·J García-SotoA Darszon
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R W SchackmannB M Shapiro
Apr 1, 1994·The Journal of General Physiology·U Klöckner, G Isenberg

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Citations

Mar 7, 2014·Pflügers Archiv : European journal of physiology·Alberto Darszon, Arturo Hernández-Cruz
Jun 3, 2014·Biochemical and Biophysical Research Communications·Takuya NishigakiAlberto Darszon
Jan 9, 2007·Fertility and Sterility·Fernando RomeroMaria Etsuko Miyamoto Oshiro
Jul 7, 2000·Developmental Biology·D H McCullohE L Chambers
Jan 11, 2002·Developmental Biology·A DarszonC L Treviño
Jul 18, 2001·Developmental Biology·M T González-MartínezA Darszon
Jan 2, 2003·The Journal of Physiology·Esmeralda Rodríguez, Alberto Darszon
Apr 2, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Anna T Neill, Victor D Vacquier
May 4, 2001·Molecular Reproduction and Development·E TostiG Gragnaniello
Oct 21, 2011·Physiological Reviews·Alberto DarszonClaudia L Treviño
Dec 24, 2014·The EMBO Journal·Reinhard SeifertTimo Strünker
Jan 5, 2002·The Journal of Biological Chemistry·Noritaka Hirohashi, Victor D Vacquier
Apr 30, 1999·Physiological Reviews·A DarszonF Espinosa

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